Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 8222 | 3' | -60.4 | NC_001978.2 | + | 3085 | 0.66 | 0.35869 |
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Target: 5'- cGCUGcCGCUgagcaCGuaCAGCAAgcGGGGCGGc -3' miRNA: 3'- -CGACuGCGAg----GCc-GUCGUU--CCCCGCC- -5' |
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| 8222 | 3' | -60.4 | NC_001978.2 | + | 8962 | 0.66 | 0.367143 |
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Target: 5'- --cGGCGUU-CGGCgaacaGGCGAGGGGCu- -3' miRNA: 3'- cgaCUGCGAgGCCG-----UCGUUCCCCGcc -5' |
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| 8222 | 3' | -60.4 | NC_001978.2 | + | 31093 | 0.66 | 0.381823 |
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Target: 5'- aCUGACGaacgCCgGGC-GCAAguaacagaccacccGGGGCGGa -3' miRNA: 3'- cGACUGCga--GG-CCGuCGUU--------------CCCCGCC- -5' |
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| 8222 | 3' | -60.4 | NC_001978.2 | + | 7203 | 0.66 | 0.384454 |
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Target: 5'- uGUUGACcauGUUCCGGCGGuCAGcGcGGGCa- -3' miRNA: 3'- -CGACUG---CGAGGCCGUC-GUU-C-CCCGcc -5' |
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| 8222 | 3' | -60.4 | NC_001978.2 | + | 3566 | 0.66 | 0.384454 |
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Target: 5'- uGCUGcCGUacuUCUGaGCGGCAA-GGGCGa -3' miRNA: 3'- -CGACuGCG---AGGC-CGUCGUUcCCCGCc -5' |
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| 8222 | 3' | -60.4 | NC_001978.2 | + | 21627 | 0.66 | 0.384454 |
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Target: 5'- uGCUGACccgaCGG-GGCuguuGGGGGCGGa -3' miRNA: 3'- -CGACUGcgagGCCgUCGu---UCCCCGCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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