Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 8222 | 3' | -60.4 | NC_001978.2 | + | 4606 | 0.71 | 0.191738 |
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Target: 5'- uGCUcGACGg-UgGGCAGCGAcGGGCGGa -3' miRNA: 3'- -CGA-CUGCgaGgCCGUCGUUcCCCGCC- -5' |
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| 8222 | 3' | -60.4 | NC_001978.2 | + | 15556 | 0.71 | 0.172404 |
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Target: 5'- --cGGCGUagUCgGGCGGC-AGGGGCGa -3' miRNA: 3'- cgaCUGCG--AGgCCGUCGuUCCCCGCc -5' |
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| 8222 | 3' | -60.4 | NC_001978.2 | + | 17336 | 0.71 | 0.167401 |
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Target: 5'- --cGGCGCUCaGGUguucuucGGCAacGGGGGCGGc -3' miRNA: 3'- cgaCUGCGAGgCCG-------UCGU--UCCCCGCC- -5' |
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| 8222 | 3' | -60.4 | NC_001978.2 | + | 15634 | 0.73 | 0.124475 |
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Target: 5'- gGCUcGACgGUUCCGGCGGCuucgcuGGGGCu- -3' miRNA: 3'- -CGA-CUG-CGAGGCCGUCGuu----CCCCGcc -5' |
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| 8222 | 3' | -60.4 | NC_001978.2 | + | 13301 | 0.75 | 0.096971 |
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Target: 5'- aGCgUGAgCGUUCCGGCGGCAAGGucGGCc- -3' miRNA: 3'- -CG-ACU-GCGAGGCCGUCGUUCC--CCGcc -5' |
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| 8222 | 3' | -60.4 | NC_001978.2 | + | 39900 | 1 | 0.001065 |
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Target: 5'- cGCUGACGCUCCGGCAGCAA-GGGCGGa -3' miRNA: 3'- -CGACUGCGAGGCCGUCGUUcCCCGCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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