Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 8222 | 3' | -60.4 | NC_001978.2 | + | 31093 | 0.66 | 0.381823 |
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Target: 5'- aCUGACGaacgCCgGGC-GCAAguaacagaccacccGGGGCGGa -3' miRNA: 3'- cGACUGCga--GG-CCGuCGUU--------------CCCCGCC- -5' |
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| 8222 | 3' | -60.4 | NC_001978.2 | + | 33006 | 0.67 | 0.347907 |
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Target: 5'- cCUGAacggcuucucaaGCUCCGuGCcgcgaauGGCAGGGGGCa- -3' miRNA: 3'- cGACUg-----------CGAGGC-CG-------UCGUUCCCCGcc -5' |
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| 8222 | 3' | -60.4 | NC_001978.2 | + | 34204 | 0.67 | 0.332568 |
|
Target: 5'- gGC--ACGCUCCGGCggacgcacacccgGGCAcgccggaAGGGGCa- -3' miRNA: 3'- -CGacUGCGAGGCCG-------------UCGU-------UCCCCGcc -5' |
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| 8222 | 3' | -60.4 | NC_001978.2 | + | 39417 | 0.7 | 0.212947 |
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Target: 5'- aGCUucaGGCGUggUUGGaCGGCAGGGGGCGcGg -3' miRNA: 3'- -CGA---CUGCGa-GGCC-GUCGUUCCCCGC-C- -5' |
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| 8222 | 3' | -60.4 | NC_001978.2 | + | 39900 | 1 | 0.001065 |
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Target: 5'- cGCUGACGCUCCGGCAGCAA-GGGCGGa -3' miRNA: 3'- -CGACUGCGAGGCCGUCGUUcCCCGCC- -5' |
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| 8222 | 3' | -60.4 | NC_001978.2 | + | 41371 | 0.67 | 0.3185 |
|
Target: 5'- --aGACGgcaauggaCUUCGGCaAGCGuccguucugaGGGGGCGGg -3' miRNA: 3'- cgaCUGC--------GAGGCCG-UCGU----------UCCCCGCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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