Results 21 - 26 of 26 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
8222 | 3' | -60.4 | NC_001978.2 | + | 33006 | 0.67 | 0.347907 |
Target: 5'- cCUGAacggcuucucaaGCUCCGuGCcgcgaauGGCAGGGGGCa- -3' miRNA: 3'- cGACUg-----------CGAGGC-CG-------UCGUUCCCCGcc -5' |
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8222 | 3' | -60.4 | NC_001978.2 | + | 1757 | 0.67 | 0.350375 |
Target: 5'- cGCUGAacguCGCacaCGGCGGCAAGcuucGGGCu- -3' miRNA: 3'- -CGACU----GCGag-GCCGUCGUUC----CCCGcc -5' |
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8222 | 3' | -60.4 | NC_001978.2 | + | 21627 | 0.66 | 0.384454 |
Target: 5'- uGCUGACccgaCGG-GGCuguuGGGGGCGGa -3' miRNA: 3'- -CGACUGcgagGCCgUCGu---UCCCCGCC- -5' |
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8222 | 3' | -60.4 | NC_001978.2 | + | 7203 | 0.66 | 0.384454 |
Target: 5'- uGUUGACcauGUUCCGGCGGuCAGcGcGGGCa- -3' miRNA: 3'- -CGACUG---CGAGGCCGUC-GUU-C-CCCGcc -5' |
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8222 | 3' | -60.4 | NC_001978.2 | + | 3566 | 0.66 | 0.384454 |
Target: 5'- uGCUGcCGUacuUCUGaGCGGCAA-GGGCGa -3' miRNA: 3'- -CGACuGCG---AGGC-CGUCGUUcCCCGCc -5' |
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8222 | 3' | -60.4 | NC_001978.2 | + | 13301 | 0.75 | 0.096971 |
Target: 5'- aGCgUGAgCGUUCCGGCGGCAAGGucGGCc- -3' miRNA: 3'- -CG-ACU-GCGAGGCCGUCGUUCC--CCGcc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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