Results 21 - 27 of 27 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 8224 | 3' | -54.5 | NC_001978.2 | + | 8871 | 0.71 | 0.405967 |
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Target: 5'- --uCGCCCc-CGAccGGCGCGCUUCGu -3' miRNA: 3'- cuuGCGGGuaGCUcuUCGCGCGAAGC- -5' |
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| 8224 | 3' | -54.5 | NC_001978.2 | + | 6418 | 0.73 | 0.294799 |
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Target: 5'- aAGCGCCCgAUCGGGAAuGCGCGacacguggUCGa -3' miRNA: 3'- cUUGCGGG-UAGCUCUU-CGCGCga------AGC- -5' |
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| 8224 | 3' | -54.5 | NC_001978.2 | + | 4905 | 0.67 | 0.595713 |
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Target: 5'- uGAGCGCCaacuuuGAGGCGCGCgagCGu -3' miRNA: 3'- -CUUGCGGguagcuCUUCGCGCGaa-GC- -5' |
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| 8224 | 3' | -54.5 | NC_001978.2 | + | 4349 | 0.69 | 0.496808 |
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Target: 5'- -uGCGCCCGUCauccGAcGCGCGCUcuUCa -3' miRNA: 3'- cuUGCGGGUAGcu--CUuCGCGCGA--AGc -5' |
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| 8224 | 3' | -54.5 | NC_001978.2 | + | 2635 | 0.66 | 0.640881 |
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Target: 5'- aGACGCCC---GGGgcGCGCGCaUCa -3' miRNA: 3'- cUUGCGGGuagCUCuuCGCGCGaAGc -5' |
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| 8224 | 3' | -54.5 | NC_001978.2 | + | 2548 | 0.71 | 0.396528 |
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Target: 5'- gGAACGCCUcaacCGGGAAGCcGUcgGCUUCGa -3' miRNA: 3'- -CUUGCGGGua--GCUCUUCG-CG--CGAAGC- -5' |
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| 8224 | 3' | -54.5 | NC_001978.2 | + | 1038 | 0.66 | 0.685908 |
|
Target: 5'- aGACuCCCGcCGAGAAGCGCGac-CGc -3' miRNA: 3'- cUUGcGGGUaGCUCUUCGCGCgaaGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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