Results 21 - 27 of 27 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
8224 | 3' | -54.5 | NC_001978.2 | + | 24288 | 0.67 | 0.629575 |
Target: 5'- -cACGCCC--CGGGcgGAGCaauGCGCUUCGu -3' miRNA: 3'- cuUGCGGGuaGCUC--UUCG---CGCGAAGC- -5' |
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8224 | 3' | -54.5 | NC_001978.2 | + | 2635 | 0.66 | 0.640881 |
Target: 5'- aGACGCCC---GGGgcGCGCGCaUCa -3' miRNA: 3'- cUUGCGGGuagCUCuuCGCGCGaAGc -5' |
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8224 | 3' | -54.5 | NC_001978.2 | + | 28578 | 0.66 | 0.652178 |
Target: 5'- --cCGUCCuUCGAgcGAAGCGCGCgggCa -3' miRNA: 3'- cuuGCGGGuAGCU--CUUCGCGCGaa-Gc -5' |
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8224 | 3' | -54.5 | NC_001978.2 | + | 25440 | 0.66 | 0.663456 |
Target: 5'- --uCGCCCAggcuGAGGCGCugggGCUUCGc -3' miRNA: 3'- cuuGCGGGUagcuCUUCGCG----CGAAGC- -5' |
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8224 | 3' | -54.5 | NC_001978.2 | + | 32321 | 0.66 | 0.685908 |
Target: 5'- aGGCGCCacgcaagacggCGUCacAGAAGCGCGCUgaagCGu -3' miRNA: 3'- cUUGCGG-----------GUAGc-UCUUCGCGCGAa---GC- -5' |
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8224 | 3' | -54.5 | NC_001978.2 | + | 40015 | 0.66 | 0.697058 |
Target: 5'- ---gGCCCuaaGUCGugguGGggGCGCGCgUCa -3' miRNA: 3'- cuugCGGG---UAGC----UCuuCGCGCGaAGc -5' |
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8224 | 3' | -54.5 | NC_001978.2 | + | 40123 | 1.09 | 0.000819 |
Target: 5'- gGAACGCCCAUCGAGAAGCGCGCUUCGa -3' miRNA: 3'- -CUUGCGGGUAGCUCUUCGCGCGAAGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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