Results 21 - 27 of 27 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 8224 | 3' | -54.5 | NC_001978.2 | + | 8871 | 0.71 | 0.405967 |
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Target: 5'- --uCGCCCc-CGAccGGCGCGCUUCGu -3' miRNA: 3'- cuuGCGGGuaGCUcuUCGCGCGAAGC- -5' |
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| 8224 | 3' | -54.5 | NC_001978.2 | + | 2548 | 0.71 | 0.396528 |
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Target: 5'- gGAACGCCUcaacCGGGAAGCcGUcgGCUUCGa -3' miRNA: 3'- -CUUGCGGGua--GCUCUUCG-CG--CGAAGC- -5' |
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| 8224 | 3' | -54.5 | NC_001978.2 | + | 13212 | 0.71 | 0.378081 |
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Target: 5'- gGAACGCCCAggcuGAAGCGUGUcggggUCGg -3' miRNA: 3'- -CUUGCGGGUagcuCUUCGCGCGa----AGC- -5' |
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| 8224 | 3' | -54.5 | NC_001978.2 | + | 19419 | 0.72 | 0.342955 |
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Target: 5'- cGGCGUCCGUCGGGAcGUaGCGCUcCGa -3' miRNA: 3'- cUUGCGGGUAGCUCUuCG-CGCGAaGC- -5' |
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| 8224 | 3' | -54.5 | NC_001978.2 | + | 6418 | 0.73 | 0.294799 |
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Target: 5'- aAGCGCCCgAUCGGGAAuGCGCGacacguggUCGa -3' miRNA: 3'- cUUGCGGG-UAGCUCUU-CGCGCga------AGC- -5' |
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| 8224 | 3' | -54.5 | NC_001978.2 | + | 40203 | 0.75 | 0.20319 |
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Target: 5'- --uCGCCCAcgUGAucGAAGCGCGCUUCu -3' miRNA: 3'- cuuGCGGGUa-GCU--CUUCGCGCGAAGc -5' |
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| 8224 | 3' | -54.5 | NC_001978.2 | + | 40123 | 1.09 | 0.000819 |
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Target: 5'- gGAACGCCCAUCGAGAAGCGCGCUUCGa -3' miRNA: 3'- -CUUGCGGGUAGCUCUUCGCGCGAAGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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