Results 1 - 8 of 8 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
8247 | 3' | -50.3 | NC_001987.1 | + | 100882 | 0.66 | 0.989985 |
Target: 5'- aUCAGGAC--ACAGGCAGC--UCCUa -3' miRNA: 3'- cGGUUCUGuuUGUCCGUUGcuAGGGg -5' |
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8247 | 3' | -50.3 | NC_001987.1 | + | 54296 | 0.66 | 0.989985 |
Target: 5'- cCCAGGcaGCAAAUAGGUaaaaacuggGACGAUgCCa -3' miRNA: 3'- cGGUUC--UGUUUGUCCG---------UUGCUAgGGg -5' |
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8247 | 3' | -50.3 | NC_001987.1 | + | 98809 | 0.66 | 0.985216 |
Target: 5'- -aCAGGaACAAGCAGGCAcaACcugCUCCg -3' miRNA: 3'- cgGUUC-UGUUUGUCCGU--UGcuaGGGG- -5' |
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8247 | 3' | -50.3 | NC_001987.1 | + | 548 | 0.67 | 0.978884 |
Target: 5'- aGUUcGGACAGugAGGaagaaaGGUCCCCu -3' miRNA: 3'- -CGGuUCUGUUugUCCguug--CUAGGGG- -5' |
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8247 | 3' | -50.3 | NC_001987.1 | + | 80902 | 0.67 | 0.976119 |
Target: 5'- aGUC-AGGCAcuucaacAAUAGGCAAagaaGAUCCUCu -3' miRNA: 3'- -CGGuUCUGU-------UUGUCCGUUg---CUAGGGG- -5' |
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8247 | 3' | -50.3 | NC_001987.1 | + | 89601 | 0.68 | 0.967575 |
Target: 5'- uCCAAGACGAGCuuGGGCAAU--UCCa- -3' miRNA: 3'- cGGUUCUGUUUG--UCCGUUGcuAGGgg -5' |
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8247 | 3' | -50.3 | NC_001987.1 | + | 43363 | 0.74 | 0.757467 |
Target: 5'- -aCAGGcACAugaagcuauuuGAUAGGUAGCGGUCCCUg -3' miRNA: 3'- cgGUUC-UGU-----------UUGUCCGUUGCUAGGGG- -5' |
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8247 | 3' | -50.3 | NC_001987.1 | + | 73914 | 1.14 | 0.003571 |
Target: 5'- gGCCAAGACAAACAGGCAACGAUCCCCc -3' miRNA: 3'- -CGGUUCUGUUUGUCCGUUGCUAGGGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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