Results 21 - 27 of 27 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
|
Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 8481 | 5' | -54 | NC_002169.1 | + | 54109 | 0.67 | 0.940942 |
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Target: 5'- gUGCCGUCccgcuGGCcaagaGACUUUUGGCCa -3' miRNA: 3'- -ACGGCAGcu---CUGug---CUGAGAACUGGg -5' |
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| 8481 | 5' | -54 | NC_002169.1 | + | 36933 | 0.66 | 0.945516 |
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Target: 5'- aGCCGaCGGGGCuuuCGGCgag-GAUCCa -3' miRNA: 3'- aCGGCaGCUCUGu--GCUGagaaCUGGG- -5' |
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| 8481 | 5' | -54 | NC_002169.1 | + | 90814 | 0.66 | 0.949852 |
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Target: 5'- cUGuuGUCGAGGCAUuuuugaGcACUCUUGaAUCUa -3' miRNA: 3'- -ACggCAGCUCUGUG------C-UGAGAAC-UGGG- -5' |
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| 8481 | 5' | -54 | NC_002169.1 | + | 128801 | 0.66 | 0.953952 |
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Target: 5'- gUGCCGUCGAGcCAUG-Cg--UGACgCu -3' miRNA: 3'- -ACGGCAGCUCuGUGCuGagaACUGgG- -5' |
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| 8481 | 5' | -54 | NC_002169.1 | + | 92780 | 0.66 | 0.956683 |
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Target: 5'- aUGUCcUCGAGcgucuugagaccgcACGCGACgcugUGGCCCa -3' miRNA: 3'- -ACGGcAGCUC--------------UGUGCUGaga-ACUGGG- -5' |
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| 8481 | 5' | -54 | NC_002169.1 | + | 43316 | 0.66 | 0.961458 |
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Target: 5'- cGUCGUUGAccaucGACACGguGCUCUUGAa-- -3' miRNA: 3'- aCGGCAGCU-----CUGUGC--UGAGAACUggg -5' |
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| 8481 | 5' | -54 | NC_002169.1 | + | 2938 | 1.13 | 0.002444 |
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Target: 5'- gUGCCGUCGAGACACGACUCUUGACCCa -3' miRNA: 3'- -ACGGCAGCUCUGUGCUGAGAACUGGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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