Results 21 - 27 of 27 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
|
R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 8481 | 5' | -54 | NC_002169.1 | + | 100953 | 0.66 | 0.957819 |
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Target: 5'- -uUCGUCGuAGACA-GACUUguaguuccuUUGGCCCa -3' miRNA: 3'- acGGCAGC-UCUGUgCUGAG---------AACUGGG- -5' |
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| 8481 | 5' | -54 | NC_002169.1 | + | 114103 | 0.66 | 0.959301 |
|
Target: 5'- aGCUGUCGAcGAgGCGACUCaauucgauaaaacGAgCCg -3' miRNA: 3'- aCGGCAGCU-CUgUGCUGAGaa-----------CUgGG- -5' |
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| 8481 | 5' | -54 | NC_002169.1 | + | 115305 | 0.67 | 0.925765 |
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Target: 5'- cGCUGUCGAGAgCAgGACacaccGAUCCg -3' miRNA: 3'- aCGGCAGCUCU-GUgCUGagaa-CUGGG- -5' |
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| 8481 | 5' | -54 | NC_002169.1 | + | 118920 | 0.8 | 0.319419 |
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Target: 5'- uUGUCGUCGGGAUuuUGGCgccgCUUGACCCg -3' miRNA: 3'- -ACGGCAGCUCUGu-GCUGa---GAACUGGG- -5' |
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| 8481 | 5' | -54 | NC_002169.1 | + | 127499 | 0.71 | 0.76341 |
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Target: 5'- --aCGUCGAGACAcCGACUCccagcGAUCCc -3' miRNA: 3'- acgGCAGCUCUGU-GCUGAGaa---CUGGG- -5' |
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| 8481 | 5' | -54 | NC_002169.1 | + | 128801 | 0.66 | 0.953952 |
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Target: 5'- gUGCCGUCGAGcCAUG-Cg--UGACgCu -3' miRNA: 3'- -ACGGCAGCUCuGUGCuGagaACUGgG- -5' |
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| 8481 | 5' | -54 | NC_002169.1 | + | 132954 | 0.66 | 0.953952 |
|
Target: 5'- aUGCCGUCuuGAagaGAUUCUUGACa- -3' miRNA: 3'- -ACGGCAGcuCUgugCUGAGAACUGgg -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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