Results 21 - 29 of 29 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 8482 | 5' | -53.8 | NC_002169.1 | + | 90235 | 0.72 | 0.688291 |
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Target: 5'- cGCCGugGuCUGACgACUaucGGCGCUGACGa -3' miRNA: 3'- aUGGCugU-GGCUG-UGA---CUGUGACUGC- -5' |
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| 8482 | 5' | -53.8 | NC_002169.1 | + | 97067 | 0.67 | 0.929686 |
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Target: 5'- cUGCCGACGgugucgaCGACGCUGcCGgUGAUGc -3' miRNA: 3'- -AUGGCUGUg------GCUGUGACuGUgACUGC- -5' |
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| 8482 | 5' | -53.8 | NC_002169.1 | + | 99394 | 0.84 | 0.168271 |
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Target: 5'- cACCacaGAUACCGACACUGACACUGAa- -3' miRNA: 3'- aUGG---CUGUGGCUGUGACUGUGACUgc -5' |
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| 8482 | 5' | -53.8 | NC_002169.1 | + | 99866 | 0.68 | 0.886557 |
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Target: 5'- -uCCGACGaugaCGACAUUGACGaguaUGAUGa -3' miRNA: 3'- auGGCUGUg---GCUGUGACUGUg---ACUGC- -5' |
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| 8482 | 5' | -53.8 | NC_002169.1 | + | 99903 | 0.67 | 0.924264 |
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Target: 5'- uUGCCGACGCCuuGAUcaaAC-GACACUGAa- -3' miRNA: 3'- -AUGGCUGUGG--CUG---UGaCUGUGACUgc -5' |
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| 8482 | 5' | -53.8 | NC_002169.1 | + | 104777 | 0.69 | 0.856709 |
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Target: 5'- cGCCGACGuuGuucguuuuGCugUGACAUUGAUa -3' miRNA: 3'- aUGGCUGUggC--------UGugACUGUGACUGc -5' |
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| 8482 | 5' | -53.8 | NC_002169.1 | + | 112939 | 0.67 | 0.929686 |
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Target: 5'- aACCGACuacgaCGACGCcGACAUUG-CGu -3' miRNA: 3'- aUGGCUGug---GCUGUGaCUGUGACuGC- -5' |
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| 8482 | 5' | -53.8 | NC_002169.1 | + | 120407 | 0.7 | 0.796648 |
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Target: 5'- aAUCGAUGuuGAUGUUGGCGCUGACGa -3' miRNA: 3'- aUGGCUGUggCUGUGACUGUGACUGC- -5' |
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| 8482 | 5' | -53.8 | NC_002169.1 | + | 129976 | 0.67 | 0.924264 |
|
Target: 5'- aGCgCGACGCgCGACAUUGucgcCAUUGugGu -3' miRNA: 3'- aUG-GCUGUG-GCUGUGACu---GUGACugC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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