Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 8576 | 5' | -50.7 | NC_002169.1 | + | 18389 | 0.66 | 0.99292 |
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Target: 5'- gGCgGCCguGAaUgGGuuAUCGACGUGGa -3' miRNA: 3'- -CGgCGGguCUaAgCUu-UAGUUGCGCC- -5' |
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| 8576 | 5' | -50.7 | NC_002169.1 | + | 53655 | 0.66 | 0.99292 |
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Target: 5'- -aCGCCggCGGAUUCG-GAUCGAC-CGGc -3' miRNA: 3'- cgGCGG--GUCUAAGCuUUAGUUGcGCC- -5' |
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| 8576 | 5' | -50.7 | NC_002169.1 | + | 53415 | 0.66 | 0.99378 |
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Target: 5'- uGCCGCCgagaauguaugcuCAGAUcaaaagccggUCGGGA--AACGCGGc -3' miRNA: 3'- -CGGCGG-------------GUCUA----------AGCUUUagUUGCGCC- -5' |
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| 8576 | 5' | -50.7 | NC_002169.1 | + | 41483 | 0.66 | 0.994634 |
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Target: 5'- aUCGCCC-GAUgccuauuUCGAAGUUAGCGUa- -3' miRNA: 3'- cGGCGGGuCUA-------AGCUUUAGUUGCGcc -5' |
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| 8576 | 5' | -50.7 | NC_002169.1 | + | 78035 | 0.66 | 0.994714 |
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Target: 5'- uCCGCCC-GA--CGAAcgcuUCGACGCGa -3' miRNA: 3'- cGGCGGGuCUaaGCUUu---AGUUGCGCc -5' |
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| 8576 | 5' | -50.7 | NC_002169.1 | + | 133809 | 0.66 | 0.994714 |
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Target: 5'- uGCCGUCaacaAGugcgUCGAcGUCGACGCc- -3' miRNA: 3'- -CGGCGGg---UCua--AGCUuUAGUUGCGcc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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