Results 1 - 5 of 5 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
8616 | 3' | -54.8 | NC_002229.2 | + | 1133 | 1.12 | 0.002732 |
Target: 5'- cGCUUAUUGCGGAGCGUGCCGAGGCAGa -3' miRNA: 3'- -CGAAUAACGCCUCGCACGGCUCCGUC- -5' |
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8616 | 3' | -54.8 | NC_002229.2 | + | 137579 | 1.12 | 0.002732 |
Target: 5'- cGCUUAUUGCGGAGCGUGCCGAGGCAGa -3' miRNA: 3'- -CGAAUAACGCCUCGCACGGCUCCGUC- -5' |
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8616 | 3' | -54.8 | NC_002229.2 | + | 27658 | 0.7 | 0.76997 |
Target: 5'- --aUAUUGCGGAacgcGUGUGCUGAGaGCGc -3' miRNA: 3'- cgaAUAACGCCU----CGCACGGCUC-CGUc -5' |
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8616 | 3' | -54.8 | NC_002229.2 | + | 172292 | 0.66 | 0.952093 |
Target: 5'- cCUUggUGCGGGGCGguuCCuAGGCAc -3' miRNA: 3'- cGAAuaACGCCUCGCac-GGcUCCGUc -5' |
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8616 | 3' | -54.8 | NC_002229.2 | + | 140322 | 0.66 | 0.952093 |
Target: 5'- cCUUggUGCGGGGCGguuCCuAGGCAc -3' miRNA: 3'- cGAAuaACGCCUCGCac-GGcUCCGUc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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