Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 8619 | 3' | -47.1 | NC_002229.2 | + | 132509 | 0.66 | 0.9999 |
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Target: 5'- gGAUCAGaaCCCUCCGUAUuAUGcAAAc -3' miRNA: 3'- -CUAGUUccGGGAGGCAUAuUACaUUU- -5' |
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| 8619 | 3' | -47.1 | NC_002229.2 | + | 146265 | 0.66 | 0.9999 |
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Target: 5'- -cUCAGGGCCUgCCGUGUuuuccggcAUGUGc- -3' miRNA: 3'- cuAGUUCCGGGaGGCAUAu-------UACAUuu -5' |
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| 8619 | 3' | -47.1 | NC_002229.2 | + | 166349 | 0.66 | 0.9999 |
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Target: 5'- -cUCAGGGCCUgCCGUGUuuuccggcAUGUGc- -3' miRNA: 3'- cuAGUUCCGGGaGGCAUAu-------UACAUuu -5' |
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| 8619 | 3' | -47.1 | NC_002229.2 | + | 6202 | 0.66 | 0.9999 |
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Target: 5'- gGAUCAGaaCCCUCCGUAUuAUGcAAAc -3' miRNA: 3'- -CUAGUUccGGGAGGCAUAuUACaUUU- -5' |
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| 8619 | 3' | -47.1 | NC_002229.2 | + | 25448 | 0.66 | 0.999829 |
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Target: 5'- aGUCAAGauauauaCCUgCGUAUGAUGUAGAc -3' miRNA: 3'- cUAGUUCcg-----GGAgGCAUAUUACAUUU- -5' |
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| 8619 | 3' | -47.1 | NC_002229.2 | + | 6488 | 0.67 | 0.999287 |
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Target: 5'- uGAUCAAGGCCUggCgGUAUAAUu---- -3' miRNA: 3'- -CUAGUUCCGGGa-GgCAUAUUAcauuu -5' |
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| 8619 | 3' | -47.1 | NC_002229.2 | + | 132223 | 0.67 | 0.999287 |
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Target: 5'- uGAUCAAGGCCUggCgGUAUAAUu---- -3' miRNA: 3'- -CUAGUUCCGGGa-GgCAUAUUAcauuu -5' |
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| 8619 | 3' | -47.1 | NC_002229.2 | + | 91459 | 0.75 | 0.915085 |
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Target: 5'- --cCAAGGUCCUCCGUAUAuguucccgcAUGUGc- -3' miRNA: 3'- cuaGUUCCGGGAGGCAUAU---------UACAUuu -5' |
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| 8619 | 3' | -47.1 | NC_002229.2 | + | 6439 | 1.06 | 0.031602 |
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Target: 5'- cGAUCAAGGCCCUCCGUAUAAUGUAAu -3' miRNA: 3'- -CUAGUUCCGGGAGGCAUAUUACAUUu -5' |
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| 8619 | 3' | -47.1 | NC_002229.2 | + | 132272 | 1.07 | 0.025704 |
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Target: 5'- cGAUCAAGGCCCUCCGUAUAAUGUAAAu -3' miRNA: 3'- -CUAGUUCCGGGAGGCAUAUUACAUUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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