miRNA: 3'- gcaaGCGUGGuCUCAGGUUAUUAUAu -5'
miRNA: 3'- -GCAAGCGUGGU-CUcaGGUUAUUAUAU- -5'
miRNA: 3'- -GCAAGCGUGGU-CUcaGGUUAUUAUAU- -5'
miRNA: 3'- -GCAAGCGUGGUCUCAGGUUAUUAUAU- -5'
miRNA: 3'- -GCAAGCGUGGUCUCAGGUUAUUAUAU- -5'
Results 1 - 5 of 5 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 8625 | 3' | -45.8 | NC_002229.2 | + | 48400 | 0.68 | 0.99984 |
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Target: 5'- ---gUGCACC-GAGUCUGAUAGUGUu -3' miRNA: 3'- gcaaGCGUGGuCUCAGGUUAUUAUAu -5' |
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| 8625 | 3' | -45.8 | NC_002229.2 | + | 12077 | 0.71 | 0.996238 |
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Target: 5'- gCGUUCGCACUGcGAa-CCAAUAAUAUAu -3' miRNA: 3'- -GCAAGCGUGGU-CUcaGGUUAUUAUAU- -5' |
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| 8625 | 3' | -45.8 | NC_002229.2 | + | 126636 | 0.71 | 0.996238 |
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Target: 5'- gCGUUCGCACUGcGAa-CCAAUAAUAUAu -3' miRNA: 3'- -GCAAGCGUGGU-CUcaGGUUAUUAUAU- -5' |
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| 8625 | 3' | -45.8 | NC_002229.2 | + | 126873 | 1.09 | 0.032123 |
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Target: 5'- gCGUUCGCACCAGAGUCCAAUAAUAUAa -3' miRNA: 3'- -GCAAGCGUGGUCUCAGGUUAUUAUAU- -5' |
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| 8625 | 3' | -45.8 | NC_002229.2 | + | 11840 | 1.09 | 0.032123 |
|
Target: 5'- gCGUUCGCACCAGAGUCCAAUAAUAUAa -3' miRNA: 3'- -GCAAGCGUGGUCUCAGGUUAUUAUAU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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