Results 1 - 20 of 24 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
8638 | 3' | -46.9 | NC_002229.2 | + | 169379 | 0.66 | 0.999933 |
Target: 5'- ---gUGCCCAuuugccGCAGGCuguacUGgGCCGg -3' miRNA: 3'- guuaAUGGGUuu----UGUUCGu----ACgCGGC- -5' |
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8638 | 3' | -46.9 | NC_002229.2 | + | 143235 | 0.66 | 0.999933 |
Target: 5'- ---gUGCCCAuuugccGCAGGCuguacUGgGCCGg -3' miRNA: 3'- guuaAUGGGUuu----UGUUCGu----ACgCGGC- -5' |
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8638 | 3' | -46.9 | NC_002229.2 | + | 67419 | 0.66 | 0.999912 |
Target: 5'- ----cGCCauuAAAgAAGCAgUGCGCCGc -3' miRNA: 3'- guuaaUGGgu-UUUgUUCGU-ACGCGGC- -5' |
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8638 | 3' | -46.9 | NC_002229.2 | + | 55033 | 0.66 | 0.999912 |
Target: 5'- ----gGCUguGAugGAGUAUGCGUCa -3' miRNA: 3'- guuaaUGGguUUugUUCGUACGCGGc -5' |
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8638 | 3' | -46.9 | NC_002229.2 | + | 143713 | 0.66 | 0.999884 |
Target: 5'- ----gGCCUuguaAAGGcCAAGCAccUGCGCCGc -3' miRNA: 3'- guuaaUGGG----UUUU-GUUCGU--ACGCGGC- -5' |
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8638 | 3' | -46.9 | NC_002229.2 | + | 168901 | 0.66 | 0.999884 |
Target: 5'- ----gGCCUuguaAAGGcCAAGCAccUGCGCCGc -3' miRNA: 3'- guuaaUGGG----UUUU-GUUCGU--ACGCGGC- -5' |
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8638 | 3' | -46.9 | NC_002229.2 | + | 29748 | 0.66 | 0.999848 |
Target: 5'- ----cGCCCuguGCGAGCAacaaaaUGUGCCu -3' miRNA: 3'- guuaaUGGGuuuUGUUCGU------ACGCGGc -5' |
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8638 | 3' | -46.9 | NC_002229.2 | + | 2877 | 0.67 | 0.999748 |
Target: 5'- cCAGUUAUgCAuGACGcGCAUGCGUaCGg -3' miRNA: 3'- -GUUAAUGgGUuUUGUuCGUACGCG-GC- -5' |
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8638 | 3' | -46.9 | NC_002229.2 | + | 135834 | 0.67 | 0.999748 |
Target: 5'- cCAGUUAUgCAuGACGcGCAUGCGUaCGg -3' miRNA: 3'- -GUUAAUGgGUuUUGUuCGUACGCG-GC- -5' |
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8638 | 3' | -46.9 | NC_002229.2 | + | 111595 | 0.67 | 0.999679 |
Target: 5'- ---aUACCCGAucguuguAUAAGCAUGCGagCGc -3' miRNA: 3'- guuaAUGGGUUu------UGUUCGUACGCg-GC- -5' |
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8638 | 3' | -46.9 | NC_002229.2 | + | 143803 | 0.67 | 0.999594 |
Target: 5'- ----gACCCAGAuCGAGCguggGUGCGCa- -3' miRNA: 3'- guuaaUGGGUUUuGUUCG----UACGCGgc -5' |
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8638 | 3' | -46.9 | NC_002229.2 | + | 168811 | 0.67 | 0.999594 |
Target: 5'- ----gACCCAGAuCGAGCguggGUGCGCa- -3' miRNA: 3'- guuaaUGGGUUUuGUUCG----UACGCGgc -5' |
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8638 | 3' | -46.9 | NC_002229.2 | + | 19848 | 0.68 | 0.999364 |
Target: 5'- ---gUGCUCGAAuauguuuAGCGUGCGCCc -3' miRNA: 3'- guuaAUGGGUUUugu----UCGUACGCGGc -5' |
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8638 | 3' | -46.9 | NC_002229.2 | + | 140101 | 0.68 | 0.999275 |
Target: 5'- gUAAUU-CCCAGAgaaaacugucauuucGCAGGgAUGCGUCGc -3' miRNA: 3'- -GUUAAuGGGUUU---------------UGUUCgUACGCGGC- -5' |
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8638 | 3' | -46.9 | NC_002229.2 | + | 172514 | 0.68 | 0.999275 |
Target: 5'- gUAAUU-CCCAGAgaaaacugucauuucGCAGGgAUGCGUCGc -3' miRNA: 3'- -GUUAAuGGGUUU---------------UGUUCgUACGCGGC- -5' |
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8638 | 3' | -46.9 | NC_002229.2 | + | 63291 | 0.69 | 0.998244 |
Target: 5'- ----cGCuCCAAAACAguuuugagAGCAucaUGCGCCGc -3' miRNA: 3'- guuaaUG-GGUUUUGU--------UCGU---ACGCGGC- -5' |
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8638 | 3' | -46.9 | NC_002229.2 | + | 158065 | 0.69 | 0.99677 |
Target: 5'- ---cUGCCCAAGACAaaccaugcuacuagAGCAucauauuUGCGUCGu -3' miRNA: 3'- guuaAUGGGUUUUGU--------------UCGU-------ACGCGGC- -5' |
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8638 | 3' | -46.9 | NC_002229.2 | + | 25516 | 0.7 | 0.995055 |
Target: 5'- gAAUUGCCCAuauC-AGUGUGUGCCu -3' miRNA: 3'- gUUAAUGGGUuuuGuUCGUACGCGGc -5' |
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8638 | 3' | -46.9 | NC_002229.2 | + | 145337 | 0.73 | 0.96667 |
Target: 5'- ----gGCCCAGAACGAGgGggacuuggGCGCCa -3' miRNA: 3'- guuaaUGGGUUUUGUUCgUa-------CGCGGc -5' |
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8638 | 3' | -46.9 | NC_002229.2 | + | 170192 | 0.73 | 0.96667 |
Target: 5'- --cUUACCCaAGAGCAGuGCGUGgGCUGg -3' miRNA: 3'- guuAAUGGG-UUUUGUU-CGUACgCGGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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