Results 1 - 11 of 11 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
8642 | 3' | -54.5 | NC_002229.2 | + | 106999 | 0.66 | 0.961625 |
Target: 5'- gAGAAugCUCUCGCuuggaaacGGaCCGcgaaACAGGCc -3' miRNA: 3'- gUUUUugGAGAGCG--------CC-GGC----UGUCCGu -5' |
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8642 | 3' | -54.5 | NC_002229.2 | + | 56193 | 0.66 | 0.945791 |
Target: 5'- aCAAAAugCUCUgGuuGCCGACAaGCc -3' miRNA: 3'- -GUUUUugGAGAgCgcCGGCUGUcCGu -5' |
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8642 | 3' | -54.5 | NC_002229.2 | + | 71880 | 0.66 | 0.941248 |
Target: 5'- --uAGGCgUCggGCGGCCGACucaauucGGCAg -3' miRNA: 3'- guuUUUGgAGagCGCCGGCUGu------CCGU- -5' |
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8642 | 3' | -54.5 | NC_002229.2 | + | 730 | 0.67 | 0.936465 |
Target: 5'- aCAGAGGCgaaCUCuUCGCGGCggcaaUGGCGGGUg -3' miRNA: 3'- -GUUUUUG---GAG-AGCGCCG-----GCUGUCCGu -5' |
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8642 | 3' | -54.5 | NC_002229.2 | + | 137981 | 0.67 | 0.936465 |
Target: 5'- aCAGAGGCgaaCUCuUCGCGGCggcaaUGGCGGGUg -3' miRNA: 3'- -GUUUUUG---GAG-AGCGCCG-----GCUGUCCGu -5' |
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8642 | 3' | -54.5 | NC_002229.2 | + | 693 | 0.69 | 0.860493 |
Target: 5'- ---cGGCCgacCGCGGCCuGCGGGCGa -3' miRNA: 3'- guuuUUGGagaGCGCCGGcUGUCCGU- -5' |
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8642 | 3' | -54.5 | NC_002229.2 | + | 138018 | 0.69 | 0.860493 |
Target: 5'- ---cGGCCgacCGCGGCCuGCGGGCGa -3' miRNA: 3'- guuuUUGGagaGCGCCGGcUGUCCGU- -5' |
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8642 | 3' | -54.5 | NC_002229.2 | + | 137426 | 0.9 | 0.071717 |
Target: 5'- -uAAAACCUCUCGCGGCCGACAGaCAg -3' miRNA: 3'- guUUUUGGAGAGCGCCGGCUGUCcGU- -5' |
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8642 | 3' | -54.5 | NC_002229.2 | + | 1285 | 0.9 | 0.071717 |
Target: 5'- -uAAAACCUCUCGCGGCCGACAGaCAg -3' miRNA: 3'- guUUUUGGAGAGCGCCGGCUGUCcGU- -5' |
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8642 | 3' | -54.5 | NC_002229.2 | + | 138905 | 1.09 | 0.004516 |
Target: 5'- cCAAAAACCUCUCGCGGCCGACAGGCAg -3' miRNA: 3'- -GUUUUUGGAGAGCGCCGGCUGUCCGU- -5' |
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8642 | 3' | -54.5 | NC_002229.2 | + | 173709 | 1.09 | 0.004516 |
Target: 5'- cCAAAAACCUCUCGCGGCCGACAGGCAg -3' miRNA: 3'- -GUUUUUGGAGAGCGCCGGCUGUCCGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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