Results 1 - 20 of 53 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
8658 | 3' | -50.4 | NC_002229.2 | + | 323 | 0.66 | 0.997288 |
Target: 5'- uGUnuGCC-GUAGCUAUuGGCg-GCGGg -3' miRNA: 3'- gCGg-CGGuUAUCGAUG-CCGunUGUCg -5' |
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8658 | 3' | -50.4 | NC_002229.2 | + | 138389 | 0.66 | 0.997288 |
Target: 5'- uGUnuGCC-GUAGCUAUuGGCg-GCGGg -3' miRNA: 3'- gCGg-CGGuUAUCGAUG-CCGunUGUCg -5' |
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8658 | 3' | -50.4 | NC_002229.2 | + | 78334 | 0.66 | 0.996792 |
Target: 5'- aGCCGgCGGaGGUUugGGCuuggaAGCc -3' miRNA: 3'- gCGGCgGUUaUCGAugCCGunug-UCG- -5' |
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8658 | 3' | -50.4 | NC_002229.2 | + | 155260 | 0.66 | 0.996224 |
Target: 5'- aGCUuGUgGAUAGUUGgGGCg-GCAGa -3' miRNA: 3'- gCGG-CGgUUAUCGAUgCCGunUGUCg -5' |
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8658 | 3' | -50.4 | NC_002229.2 | + | 75025 | 0.66 | 0.994838 |
Target: 5'- aGCUGCCAAcucuguagGGCgguUGGCc-AUGGCc -3' miRNA: 3'- gCGGCGGUUa-------UCGau-GCCGunUGUCG- -5' |
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8658 | 3' | -50.4 | NC_002229.2 | + | 146687 | 0.67 | 0.994005 |
Target: 5'- cCGuuGCCAAacGaucccagaGGCAgaGCAGCg -3' miRNA: 3'- -GCggCGGUUauCgaug----CCGUn-UGUCG- -5' |
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8658 | 3' | -50.4 | NC_002229.2 | + | 18916 | 0.67 | 0.993916 |
Target: 5'- uGaCCGCCAAUaaaauauuuuuuaAGCU-CGGgGggAUGGCa -3' miRNA: 3'- gC-GGCGGUUA-------------UCGAuGCCgUn-UGUCG- -5' |
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8658 | 3' | -50.4 | NC_002229.2 | + | 655 | 0.67 | 0.993455 |
Target: 5'- gCGCCGUgGGgcGCgcggACGGCGucgcucccacacgcGCGGCc -3' miRNA: 3'- -GCGGCGgUUauCGa---UGCCGUn-------------UGUCG- -5' |
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8658 | 3' | -50.4 | NC_002229.2 | + | 138057 | 0.67 | 0.993455 |
Target: 5'- gCGCCGUgGGgcGCgcggACGGCGucgcucccacacgcGCGGCc -3' miRNA: 3'- -GCGGCGgUUauCGa---UGCCGUn-------------UGUCG- -5' |
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8658 | 3' | -50.4 | NC_002229.2 | + | 743 | 0.67 | 0.989533 |
Target: 5'- -cCCGCCGA-GGCUGacagaGGCGaacucuucGCGGCg -3' miRNA: 3'- gcGGCGGUUaUCGAUg----CCGUn-------UGUCG- -5' |
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8658 | 3' | -50.4 | NC_002229.2 | + | 137968 | 0.67 | 0.989533 |
Target: 5'- -cCCGCCGA-GGCUGacagaGGCGaacucuucGCGGCg -3' miRNA: 3'- gcGGCGGUUaUCGAUg----CCGUn-------UGUCG- -5' |
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8658 | 3' | -50.4 | NC_002229.2 | + | 18166 | 0.68 | 0.988681 |
Target: 5'- gCGCCuGCCGAUAuuucauGCUAUGGCucaacuagacuugACAGg -3' miRNA: 3'- -GCGG-CGGUUAU------CGAUGCCGun-----------UGUCg -5' |
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8658 | 3' | -50.4 | NC_002229.2 | + | 165928 | 0.68 | 0.988085 |
Target: 5'- cCGuuGCCAAacgaucccAGUa--GGCAgaGCAGCg -3' miRNA: 3'- -GCggCGGUUa-------UCGaugCCGUn-UGUCG- -5' |
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8658 | 3' | -50.4 | NC_002229.2 | + | 106628 | 0.68 | 0.988085 |
Target: 5'- aCGauaUGCCAuuGUGGCUAUGuuGCAaGCAGa -3' miRNA: 3'- -GCg--GCGGU--UAUCGAUGC--CGUnUGUCg -5' |
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8658 | 3' | -50.4 | NC_002229.2 | + | 153369 | 0.68 | 0.986486 |
Target: 5'- uGCUGCauuccccuUAGUAGCgACGGCAcgACAu- -3' miRNA: 3'- gCGGCG--------GUUAUCGaUGCCGUn-UGUcg -5' |
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8658 | 3' | -50.4 | NC_002229.2 | + | 139270 | 0.68 | 0.984728 |
Target: 5'- uGCCGCCAAacuuguGCcaaACGGaCGggUAGCg -3' miRNA: 3'- gCGGCGGUUau----CGa--UGCC-GUnuGUCG- -5' |
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8658 | 3' | -50.4 | NC_002229.2 | + | 173344 | 0.68 | 0.984728 |
Target: 5'- uGCCGCCAAacuuguGCcaaACGGaCGggUAGCg -3' miRNA: 3'- gCGGCGGUUau----CGa--UGCC-GUnuGUCG- -5' |
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8658 | 3' | -50.4 | NC_002229.2 | + | 166913 | 0.68 | 0.982801 |
Target: 5'- gGCCGCgGAgAGCgcaGGag-GCAGCg -3' miRNA: 3'- gCGGCGgUUaUCGaugCCgunUGUCG- -5' |
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8658 | 3' | -50.4 | NC_002229.2 | + | 84206 | 0.68 | 0.982801 |
Target: 5'- aCGUCGCUcg-AGCUgGCGGUa-AguGCu -3' miRNA: 3'- -GCGGCGGuuaUCGA-UGCCGunUguCG- -5' |
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8658 | 3' | -50.4 | NC_002229.2 | + | 43736 | 0.69 | 0.978404 |
Target: 5'- cCGUCgGCCGAUauaugaaugAGUggGCGGCAauCAGUa -3' miRNA: 3'- -GCGG-CGGUUA---------UCGa-UGCCGUnuGUCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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