Results 1 - 8 of 8 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
8695 | 3' | -51.5 | NC_002229.2 | + | 150434 | 1.1 | 0.0073 |
Target: 5'- gAACCGUGCCUUUCUCUACGCAGAUGGg -3' miRNA: 3'- -UUGGCACGGAAAGAGAUGCGUCUACC- -5' |
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8695 | 3' | -51.5 | NC_002229.2 | + | 150487 | 0.73 | 0.797378 |
Target: 5'- gAACCGUGCCUUcCUCcGCGCaaauAGGUGc -3' miRNA: 3'- -UUGGCACGGAAaGAGaUGCG----UCUACc -5' |
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8695 | 3' | -51.5 | NC_002229.2 | + | 130830 | 0.7 | 0.919887 |
Target: 5'- aAACCGUGCCgacugcgUUUUUAUGUGGggGGg -3' miRNA: 3'- -UUGGCACGGaa-----AGAGAUGCGUCuaCC- -5' |
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8695 | 3' | -51.5 | NC_002229.2 | + | 779 | 0.68 | 0.96186 |
Target: 5'- cGCuCGaGCCcggUCcCUGCGCAGGUGGu -3' miRNA: 3'- uUG-GCaCGGaa-AGaGAUGCGUCUACC- -5' |
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8695 | 3' | -51.5 | NC_002229.2 | + | 137933 | 0.68 | 0.96186 |
Target: 5'- cGCuCGaGCCcggUCcCUGCGCAGGUGGu -3' miRNA: 3'- uUG-GCaCGGaa-AGaGAUGCGUCUACC- -5' |
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8695 | 3' | -51.5 | NC_002229.2 | + | 7881 | 0.7 | 0.919887 |
Target: 5'- aAACCGUGCCgacugcgUUUUUAUGUGGggGGg -3' miRNA: 3'- -UUGGCACGGaa-----AGAGAUGCGUCuaCC- -5' |
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8695 | 3' | -51.5 | NC_002229.2 | + | 162127 | 0.71 | 0.894717 |
Target: 5'- gAACCGUGCCUUcCUCcGCGCAa---- -3' miRNA: 3'- -UUGGCACGGAAaGAGaUGCGUcuacc -5' |
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8695 | 3' | -51.5 | NC_002229.2 | + | 162180 | 1.1 | 0.0073 |
Target: 5'- gAACCGUGCCUUUCUCUACGCAGAUGGg -3' miRNA: 3'- -UUGGCACGGAAAGAGAUGCGUCUACC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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