Results 1 - 7 of 7 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
8703 | 3' | -56.5 | NC_002229.2 | + | 139218 | 1.06 | 0.003889 |
Target: 5'- cCUCUACAUGACCGCGCAUGGGCCGAAa -3' miRNA: 3'- -GAGAUGUACUGGCGCGUACCCGGCUU- -5' |
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8703 | 3' | -56.5 | NC_002229.2 | + | 173396 | 1.06 | 0.003889 |
Target: 5'- cCUCUACAUGACCGCGCAUGGGCCGAAa -3' miRNA: 3'- -GAGAUGUACUGGCGCGUACCCGGCUU- -5' |
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8703 | 3' | -56.5 | NC_002229.2 | + | 173449 | 0.98 | 0.01513 |
Target: 5'- cCUCUACAUGACCGCGCAUccGGGCCGAAa -3' miRNA: 3'- -GAGAUGUACUGGCGCGUA--CCCGGCUU- -5' |
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8703 | 3' | -56.5 | NC_002229.2 | + | 139165 | 0.96 | 0.018869 |
Target: 5'- cCUCUACAUGACCGCGCAccGGGCCGAAa -3' miRNA: 3'- -GAGAUGUACUGGCGCGUa-CCCGGCUU- -5' |
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8703 | 3' | -56.5 | NC_002229.2 | + | 126264 | 0.66 | 0.885112 |
Target: 5'- ---gGCGgagGGCCGCGCAgGGGUCGc- -3' miRNA: 3'- gagaUGUa--CUGGCGCGUaCCCGGCuu -5' |
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8703 | 3' | -56.5 | NC_002229.2 | + | 67460 | 0.66 | 0.885112 |
Target: 5'- --gUACAUGGcCCGCGUGgcaGGGCUGGu -3' miRNA: 3'- gagAUGUACU-GGCGCGUa--CCCGGCUu -5' |
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8703 | 3' | -56.5 | NC_002229.2 | + | 12448 | 0.66 | 0.885112 |
Target: 5'- ---gGCGgagGGCCGCGCAgGGGUCGc- -3' miRNA: 3'- gagaUGUa--CUGGCGCGUaCCCGGCuu -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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