miRNA display CGI


Results 1 - 7 of 7 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
8703 3' -56.5 NC_002229.2 + 12448 0.66 0.885112
Target:  5'- ---gGCGgagGGCCGCGCAgGGGUCGc- -3'
miRNA:   3'- gagaUGUa--CUGGCGCGUaCCCGGCuu -5'
8703 3' -56.5 NC_002229.2 + 67460 0.66 0.885112
Target:  5'- --gUACAUGGcCCGCGUGgcaGGGCUGGu -3'
miRNA:   3'- gagAUGUACU-GGCGCGUa--CCCGGCUu -5'
8703 3' -56.5 NC_002229.2 + 126264 0.66 0.885112
Target:  5'- ---gGCGgagGGCCGCGCAgGGGUCGc- -3'
miRNA:   3'- gagaUGUa--CUGGCGCGUaCCCGGCuu -5'
8703 3' -56.5 NC_002229.2 + 139165 0.96 0.018869
Target:  5'- cCUCUACAUGACCGCGCAccGGGCCGAAa -3'
miRNA:   3'- -GAGAUGUACUGGCGCGUa-CCCGGCUU- -5'
8703 3' -56.5 NC_002229.2 + 139218 1.06 0.003889
Target:  5'- cCUCUACAUGACCGCGCAUGGGCCGAAa -3'
miRNA:   3'- -GAGAUGUACUGGCGCGUACCCGGCUU- -5'
8703 3' -56.5 NC_002229.2 + 173396 1.06 0.003889
Target:  5'- cCUCUACAUGACCGCGCAUGGGCCGAAa -3'
miRNA:   3'- -GAGAUGUACUGGCGCGUACCCGGCUU- -5'
8703 3' -56.5 NC_002229.2 + 173449 0.98 0.01513
Target:  5'- cCUCUACAUGACCGCGCAUccGGGCCGAAa -3'
miRNA:   3'- -GAGAUGUACUGGCGCGUA--CCCGGCUU- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.