Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 8703 | 5' | -57.9 | NC_002229.2 | + | 139183 | 1.06 | 0.003236 |
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Target: 5'- aACUCCGACUGCGCACGGCCUCUACAUg -3' miRNA: 3'- -UGAGGCUGACGCGUGCCGGAGAUGUA- -5' |
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| 8703 | 5' | -57.9 | NC_002229.2 | + | 173431 | 1.06 | 0.003236 |
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Target: 5'- aACUCCGACUGCGCACGGCCUCUACAUg -3' miRNA: 3'- -UGAGGCUGACGCGUGCCGGAGAUGUA- -5' |
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| 8703 | 5' | -57.9 | NC_002229.2 | + | 139129 | 0.97 | 0.013145 |
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Target: 5'- aACUCCGACUGCGCACGGCCUCUAa-- -3' miRNA: 3'- -UGAGGCUGACGCGUGCCGGAGAUgua -5' |
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| 8703 | 5' | -57.9 | NC_002229.2 | + | 173486 | 0.97 | 0.013145 |
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Target: 5'- aACUCCGACUGCGCACGGCCUCUAa-- -3' miRNA: 3'- -UGAGGCUGACGCGUGCCGGAGAUgua -5' |
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| 8703 | 5' | -57.9 | NC_002229.2 | + | 143500 | 0.73 | 0.46687 |
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Target: 5'- aACUCCG-CUGUGCGgcgcaggugcuUGGCCUUUACAa -3' miRNA: 3'- -UGAGGCuGACGCGU-----------GCCGGAGAUGUa -5' |
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| 8703 | 5' | -57.9 | NC_002229.2 | + | 169114 | 0.67 | 0.799442 |
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Target: 5'- aACUCCG-CUGUGCGgCGcaggugcuuGCCUUUACAa -3' miRNA: 3'- -UGAGGCuGACGCGU-GC---------CGGAGAUGUa -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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