Results 21 - 29 of 29 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position |
R_P_ratio #
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P value |
| Predicted miRNA align pattern | |||||||
| 8728 | 3' | -40.5 | NC_002331.1 | + | 69412 | 0.68 | 1 |
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Target: 5'- gCGauAAUUUUAAUGUCGAucuuuucgccUCCCGGAg -3' miRNA: 3'- gGUauUUAAAAUUACAGCU----------GGGGUUU- -5' |
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| 8728 | 3' | -40.5 | NC_002331.1 | + | 4035 | 0.67 | 1 |
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Target: 5'- uCCAUAGc----AAUGUUGuCCCCGAAa -3' miRNA: 3'- -GGUAUUuaaaaUUACAGCuGGGGUUU- -5' |
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| 8728 | 3' | -40.5 | NC_002331.1 | + | 3875 | 0.67 | 1 |
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Target: 5'- uCCGUGAAgcUUGcgGUCGuCCgCAAAc -3' miRNA: 3'- -GGUAUUUaaAAUuaCAGCuGGgGUUU- -5' |
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| 8728 | 3' | -40.5 | NC_002331.1 | + | 171739 | 0.67 | 1 |
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Target: 5'- uUCAUuuuAUUUUAAUGUCGAUUUCGu- -3' miRNA: 3'- -GGUAuu-UAAAAUUACAGCUGGGGUuu -5' |
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| 8728 | 3' | -40.5 | NC_002331.1 | + | 9917 | 0.66 | 1 |
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Target: 5'- aCCAUAucggc-GAUGUUGACCaCUAGAu -3' miRNA: 3'- -GGUAUuuaaaaUUACAGCUGG-GGUUU- -5' |
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| 8728 | 3' | -40.5 | NC_002331.1 | + | 37356 | 0.66 | 1 |
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Target: 5'- -gGUAAAaaaUUUAAUGUCGACCUUu-- -3' miRNA: 3'- ggUAUUUa--AAAUUACAGCUGGGGuuu -5' |
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| 8728 | 3' | -40.5 | NC_002331.1 | + | 19652 | 0.66 | 1 |
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Target: 5'- ---cGGGUUUUAAUaUCGACCUCAc- -3' miRNA: 3'- gguaUUUAAAAUUAcAGCUGGGGUuu -5' |
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| 8728 | 3' | -40.5 | NC_002331.1 | + | 46708 | 0.66 | 1 |
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Target: 5'- aCAcuuuUUUUAAUGUCGACCUUu-- -3' miRNA: 3'- gGUauuuAAAAUUACAGCUGGGGuuu -5' |
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| 8728 | 3' | -40.5 | NC_002331.1 | + | 12315 | 0.66 | 1 |
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Target: 5'- uCCGUAGAaa-UGGUGUCGAUuugaCCCGc- -3' miRNA: 3'- -GGUAUUUaaaAUUACAGCUG----GGGUuu -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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