Results 21 - 29 of 29 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 8728 | 3' | -40.5 | NC_002331.1 | + | 167702 | 0.72 | 0.999997 |
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Target: 5'- cCCAUGAccacUUGAUGU-GACCCCAu- -3' miRNA: 3'- -GGUAUUuaa-AAUUACAgCUGGGGUuu -5' |
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| 8728 | 3' | -40.5 | NC_002331.1 | + | 171571 | 0.73 | 0.999994 |
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Target: 5'- gUCAaAAAUUUUAAUGUCGAucuuuucgccUCCCGGAg -3' miRNA: 3'- -GGUaUUUAAAAUUACAGCU----------GGGGUUU- -5' |
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| 8728 | 3' | -40.5 | NC_002331.1 | + | 69033 | 0.74 | 0.999974 |
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Target: 5'- aCAcuuuUUUUAAUGUCGAUCCCGu- -3' miRNA: 3'- gGUauuuAAAAUUACAGCUGGGGUuu -5' |
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| 8728 | 3' | -40.5 | NC_002331.1 | + | 146263 | 0.76 | 0.999797 |
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Target: 5'- -gGUAAAauUUUUAAUGUCGACCCa--- -3' miRNA: 3'- ggUAUUU--AAAAUUACAGCUGGGguuu -5' |
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| 8728 | 3' | -40.5 | NC_002331.1 | + | 146628 | 0.77 | 0.99944 |
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Target: 5'- uCCAUAugguaaaAAUUUUAAUGUCGACCUUu-- -3' miRNA: 3'- -GGUAU-------UUAAAAUUACAGCUGGGGuuu -5' |
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| 8728 | 3' | -40.5 | NC_002331.1 | + | 171321 | 0.77 | 0.999297 |
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Target: 5'- gCUAaAAAUUUUAAUGUCGAucuuuuuCCCCGGAa -3' miRNA: 3'- -GGUaUUUAAAAUUACAGCU-------GGGGUUU- -5' |
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| 8728 | 3' | -40.5 | NC_002331.1 | + | 171446 | 0.8 | 0.994275 |
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Target: 5'- gCCAaAAAUUUUAAUGUCGAUcuuuuuaCCCAAAa -3' miRNA: 3'- -GGUaUUUAAAAUUACAGCUG-------GGGUUU- -5' |
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| 8728 | 3' | -40.5 | NC_002331.1 | + | 111426 | 0.85 | 0.927153 |
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Target: 5'- gCAUuuucUUUAAUGUCGACCCCAAGu -3' miRNA: 3'- gGUAuuuaAAAUUACAGCUGGGGUUU- -5' |
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| 8728 | 3' | -40.5 | NC_002331.1 | + | 111680 | 1.11 | 0.093469 |
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Target: 5'- gCCAUAAAUUUUAAUGUCGACCCCAAAg -3' miRNA: 3'- -GGUAUUUAAAAUUACAGCUGGGGUUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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