Results 1 - 5 of 5 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
8795 | 3' | -50.2 | NC_002484.1 | + | 3187 | 1.09 | 0.003199 |
Target: 5'- aUCUGCAAAUCUCGAUCACGCCAUGCUc -3' miRNA: 3'- -AGACGUUUAGAGCUAGUGCGGUACGA- -5' |
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8795 | 3' | -50.2 | NC_002484.1 | + | 25367 | 0.68 | 0.830914 |
Target: 5'- cUUGCGAAgcggCUCGA-CACGuUCAUGCg -3' miRNA: 3'- aGACGUUUa---GAGCUaGUGC-GGUACGa -5' |
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8795 | 3' | -50.2 | NC_002484.1 | + | 41916 | 0.67 | 0.890562 |
Target: 5'- cCUGCuugcaUCGAUCAaGUCAUGCg -3' miRNA: 3'- aGACGuuuagAGCUAGUgCGGUACGa -5' |
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8795 | 3' | -50.2 | NC_002484.1 | + | 6174 | 0.66 | 0.930688 |
Target: 5'- cCUGUAGugCUCGAUgCGCGCCGU-CUu -3' miRNA: 3'- aGACGUUuaGAGCUA-GUGCGGUAcGA- -5' |
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8795 | 3' | -50.2 | NC_002484.1 | + | 17895 | 0.66 | 0.936323 |
Target: 5'- --gGCucGUCUCGGgccgcagagaUCACGCCGcGCUu -3' miRNA: 3'- agaCGuuUAGAGCU----------AGUGCGGUaCGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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