miRNA display CGI


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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
8795 3' -50.2 NC_002484.1 + 3187 1.09 0.003199
Target:  5'- aUCUGCAAAUCUCGAUCACGCCAUGCUc -3'
miRNA:   3'- -AGACGUUUAGAGCUAGUGCGGUACGA- -5'
8795 3' -50.2 NC_002484.1 + 6174 0.66 0.930688
Target:  5'- cCUGUAGugCUCGAUgCGCGCCGU-CUu -3'
miRNA:   3'- aGACGUUuaGAGCUA-GUGCGGUAcGA- -5'
8795 3' -50.2 NC_002484.1 + 17895 0.66 0.936323
Target:  5'- --gGCucGUCUCGGgccgcagagaUCACGCCGcGCUu -3'
miRNA:   3'- agaCGuuUAGAGCU----------AGUGCGGUaCGA- -5'
8795 3' -50.2 NC_002484.1 + 25367 0.68 0.830914
Target:  5'- cUUGCGAAgcggCUCGA-CACGuUCAUGCg -3'
miRNA:   3'- aGACGUUUa---GAGCUaGUGC-GGUACGa -5'
8795 3' -50.2 NC_002484.1 + 41916 0.67 0.890562
Target:  5'- cCUGCuugcaUCGAUCAaGUCAUGCg -3'
miRNA:   3'- aGACGuuuagAGCUAGUgCGGUACGa -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.