Results 1 - 10 of 10 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
8799 | 3' | -51.1 | NC_002484.1 | + | 45858 | 0.66 | 0.895239 |
Target: 5'- cGGCgaaaUGAacAGCAauAAGGAGGCucuuccaggaGCUCCGa -3' miRNA: 3'- aCCGg---ACU--UCGU--UUUCUUCG----------UGAGGC- -5' |
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8799 | 3' | -51.1 | NC_002484.1 | + | 226 | 0.67 | 0.84604 |
Target: 5'- gGGCCUu--GCAGAuGGAugGGCGCUUCGc -3' miRNA: 3'- aCCGGAcuuCGUUU-UCU--UCGUGAGGC- -5' |
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8799 | 3' | -51.1 | NC_002484.1 | + | 31936 | 0.67 | 0.836936 |
Target: 5'- cGGCCUGAcggucAGCGAGGGGcuuuugcugGGUAUggCCGc -3' miRNA: 3'- aCCGGACU-----UCGUUUUCU---------UCGUGa-GGC- -5' |
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8799 | 3' | -51.1 | NC_002484.1 | + | 43072 | 0.69 | 0.745835 |
Target: 5'- cGGCC-GAAGCGAucaAGGCGCUugCCGa -3' miRNA: 3'- aCCGGaCUUCGUUuucUUCGUGA--GGC- -5' |
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8799 | 3' | -51.1 | NC_002484.1 | + | 1414 | 0.69 | 0.730518 |
Target: 5'- uUGGCCUGcugGAGCugggccaGGAGGAAGUcagggaauaccgacACUCCGu -3' miRNA: 3'- -ACCGGAC---UUCG-------UUUUCUUCG--------------UGAGGC- -5' |
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8799 | 3' | -51.1 | NC_002484.1 | + | 32734 | 0.7 | 0.667506 |
Target: 5'- aUGGCgaGAAGUGGuGGGAGCAC-CUGg -3' miRNA: 3'- -ACCGgaCUUCGUUuUCUUCGUGaGGC- -5' |
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8799 | 3' | -51.1 | NC_002484.1 | + | 42868 | 0.72 | 0.564672 |
Target: 5'- uUGGCUUGAAGgAAgcGAAGUAUgggCCGg -3' miRNA: 3'- -ACCGGACUUCgUUuuCUUCGUGa--GGC- -5' |
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8799 | 3' | -51.1 | NC_002484.1 | + | 55253 | 0.73 | 0.486482 |
Target: 5'- cGGCUUGGuggccacAGCAGAGGucgcGCGCUCCa -3' miRNA: 3'- aCCGGACU-------UCGUUUUCuu--CGUGAGGc -5' |
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8799 | 3' | -51.1 | NC_002484.1 | + | 32427 | 0.74 | 0.456018 |
Target: 5'- aGGCCUGAucaAGCAGAAcAGGCGCcUCGg -3' miRNA: 3'- aCCGGACU---UCGUUUUcUUCGUGaGGC- -5' |
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8799 | 3' | -51.1 | NC_002484.1 | + | 6671 | 1.1 | 0.001959 |
Target: 5'- cUGGCCUGAAGCAAAAGAAGCACUCCGa -3' miRNA: 3'- -ACCGGACUUCGUUUUCUUCGUGAGGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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