Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 8800 | 5' | -57.9 | NC_002484.1 | + | 15750 | 0.66 | 0.58236 |
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Target: 5'- gCCCUCuguAGGCCGCAgCGAUGaccccaggcccUGGC-UCg -3' miRNA: 3'- -GGGGG---UCCGGCGUaGCUAU-----------GCCGuAG- -5' |
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| 8800 | 5' | -57.9 | NC_002484.1 | + | 11359 | 0.66 | 0.561086 |
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Target: 5'- gUCCCUAGGCUGCGU-GAUGCcauuaccgGGC-UCc -3' miRNA: 3'- -GGGGGUCCGGCGUAgCUAUG--------CCGuAG- -5' |
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| 8800 | 5' | -57.9 | NC_002484.1 | + | 438 | 0.67 | 0.54003 |
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Target: 5'- uUUCCAGGCCGaacuucuggcgGUCGG-ACGGCGUUa -3' miRNA: 3'- gGGGGUCCGGCg----------UAGCUaUGCCGUAG- -5' |
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| 8800 | 5' | -57.9 | NC_002484.1 | + | 5189 | 0.68 | 0.439764 |
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Target: 5'- aCCagcaUCAGGCCGUAaCGAgcGCGcGCAUCg -3' miRNA: 3'- -GGg---GGUCCGGCGUaGCUa-UGC-CGUAG- -5' |
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| 8800 | 5' | -57.9 | NC_002484.1 | + | 35838 | 0.69 | 0.420986 |
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Target: 5'- gUCCCAuccGCCGacuUCGAUGCGGCAc- -3' miRNA: 3'- gGGGGUc--CGGCgu-AGCUAUGCCGUag -5' |
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| 8800 | 5' | -57.9 | NC_002484.1 | + | 17409 | 0.76 | 0.151611 |
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Target: 5'- -aCUCGGGCCGCAUCauGUACGGCAg- -3' miRNA: 3'- ggGGGUCCGGCGUAGc-UAUGCCGUag -5' |
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| 8800 | 5' | -57.9 | NC_002484.1 | + | 6755 | 1.12 | 0.000361 |
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Target: 5'- aCCCCCAGGCCGCAUCGAUACGGCAUCa -3' miRNA: 3'- -GGGGGUCCGGCGUAGCUAUGCCGUAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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