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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 8801 | 3' | -58.6 | NC_002484.1 | + | 317 | 0.67 | 0.515128 |
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Target: 5'- gUUCGCGGugaccuccaggaugaUCCGGUCCGaucccGUCAGAACg -3' miRNA: 3'- uGAGCGCU---------------GGGCCGGGU-----CAGUCUUGg -5' |
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| 8801 | 3' | -58.6 | NC_002484.1 | + | 6917 | 1.11 | 0.000409 |
|
Target: 5'- aACUCGCGACCCGGCCCAGUCAGAACCu -3' miRNA: 3'- -UGAGCGCUGGGCCGGGUCAGUCUUGG- -5' |
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| 8801 | 3' | -58.6 | NC_002484.1 | + | 14243 | 0.67 | 0.511056 |
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Target: 5'- uACUCGgGcagaauCUCGGCCCAccCAGGAUCg -3' miRNA: 3'- -UGAGCgCu-----GGGCCGGGUcaGUCUUGG- -5' |
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| 8801 | 3' | -58.6 | NC_002484.1 | + | 14349 | 0.66 | 0.58391 |
|
Target: 5'- -aUCGgaaGAUCCGGCCCA-UCcgaugcauAGAACCg -3' miRNA: 3'- ugAGCg--CUGGGCCGGGUcAG--------UCUUGG- -5' |
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| 8801 | 3' | -58.6 | NC_002484.1 | + | 19096 | 0.7 | 0.3221 |
|
Target: 5'- cGCUCGCGAuagccgaucauCCCGGCgCCAGcgCuGGugGCCa -3' miRNA: 3'- -UGAGCGCU-----------GGGCCG-GGUCa-GuCU--UGG- -5' |
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| 8801 | 3' | -58.6 | NC_002484.1 | + | 38248 | 0.71 | 0.278608 |
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Target: 5'- aACUCGaCGACCUGGUgaugCCGGcCGGcGCCg -3' miRNA: 3'- -UGAGC-GCUGGGCCG----GGUCaGUCuUGG- -5' |
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| 8801 | 3' | -58.6 | NC_002484.1 | + | 40986 | 0.67 | 0.500936 |
|
Target: 5'- cCUCGCcGCCgaGGCCCAGgcgcUCAGGgaggaagucgcaGCCg -3' miRNA: 3'- uGAGCGcUGGg-CCGGGUC----AGUCU------------UGG- -5' |
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| 8801 | 3' | -58.6 | NC_002484.1 | + | 50760 | 0.69 | 0.370468 |
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Target: 5'- uUUCGCGAUCUGcCUCGGUCgccGGGACCa -3' miRNA: 3'- uGAGCGCUGGGCcGGGUCAG---UCUUGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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