Results 1 - 11 of 11 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
8803 | 5' | -61.3 | NC_002484.1 | + | 32549 | 0.66 | 0.392493 |
Target: 5'- gCCGCCGCGCCCggcaaggAgGUaGGUCaUGAGUg -3' miRNA: 3'- -GGCGGCGCGGG-------UgCAaCCGGcGCUUA- -5' |
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8803 | 5' | -61.3 | NC_002484.1 | + | 17019 | 0.66 | 0.39076 |
Target: 5'- aCGCCGUgauccgauagcgcaGCCCACGcgGGCCGa---- -3' miRNA: 3'- gGCGGCG--------------CGGGUGCaaCCGGCgcuua -5' |
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8803 | 5' | -61.3 | NC_002484.1 | + | 14999 | 0.67 | 0.367849 |
Target: 5'- cCCGCCacCGCCCGCG-UGGCUcUGAc- -3' miRNA: 3'- -GGCGGc-GCGGGUGCaACCGGcGCUua -5' |
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8803 | 5' | -61.3 | NC_002484.1 | + | 47867 | 0.67 | 0.359601 |
Target: 5'- cUCGagaCGCGgaaCCACGUUGGCgcCGCGAu- -3' miRNA: 3'- -GGCg--GCGCg--GGUGCAACCG--GCGCUua -5' |
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8803 | 5' | -61.3 | NC_002484.1 | + | 15552 | 0.67 | 0.359601 |
Target: 5'- cCCGCC-CGCCCuCGguauagaUGGCgGUGAGg -3' miRNA: 3'- -GGCGGcGCGGGuGCa------ACCGgCGCUUa -5' |
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8803 | 5' | -61.3 | NC_002484.1 | + | 32912 | 0.67 | 0.342704 |
Target: 5'- aUCGCUGCucagcgcgacgccGCCCugGccagGGUCGCGGAg -3' miRNA: 3'- -GGCGGCG-------------CGGGugCaa--CCGGCGCUUa -5' |
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8803 | 5' | -61.3 | NC_002484.1 | + | 36727 | 0.69 | 0.284353 |
Target: 5'- gCCGCagaGCGCCaACGgcaGGCCGaCGAGc -3' miRNA: 3'- -GGCGg--CGCGGgUGCaa-CCGGC-GCUUa -5' |
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8803 | 5' | -61.3 | NC_002484.1 | + | 47949 | 0.69 | 0.251649 |
Target: 5'- aCGCCGUGuCCCGCcuccaGGCUGUGAAg -3' miRNA: 3'- gGCGGCGC-GGGUGcaa--CCGGCGCUUa -5' |
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8803 | 5' | -61.3 | NC_002484.1 | + | 47132 | 0.7 | 0.233553 |
Target: 5'- aCGUCGCGgCgCAUGUUGGUCaGCGAGc -3' miRNA: 3'- gGCGGCGCgG-GUGCAACCGG-CGCUUa -5' |
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8803 | 5' | -61.3 | NC_002484.1 | + | 41672 | 0.73 | 0.135556 |
Target: 5'- gCCGCCGCGCCCGgcaaggagggggUGUgaUGGCCGUu--- -3' miRNA: 3'- -GGCGGCGCGGGU------------GCA--ACCGGCGcuua -5' |
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8803 | 5' | -61.3 | NC_002484.1 | + | 7451 | 1.07 | 0.00042 |
Target: 5'- aCCGCCGCGCCCACGUUGGCCGCGAAUc -3' miRNA: 3'- -GGCGGCGCGGGUGCAACCGGCGCUUA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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