Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 8804 | 5' | -53.9 | NC_002484.1 | + | 54869 | 0.69 | 0.550109 |
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Target: 5'- cGAGCAGgauCGCCAGCAccagggUCAGCCa -3' miRNA: 3'- -CUCGUC---GCGGUCGUcaaca-AGUUGGc -5' |
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| 8804 | 5' | -53.9 | NC_002484.1 | + | 35554 | 0.7 | 0.506751 |
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Target: 5'- uAGCAGCGCCAGgGGUcacaagcUUgAGCCGg -3' miRNA: 3'- cUCGUCGCGGUCgUCAac-----AAgUUGGC- -5' |
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| 8804 | 5' | -53.9 | NC_002484.1 | + | 34338 | 0.67 | 0.673176 |
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Target: 5'- aAGCAGguaGaCCAGCAGUUGUagcgCGACgGg -3' miRNA: 3'- cUCGUCg--C-GGUCGUCAACAa---GUUGgC- -5' |
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| 8804 | 5' | -53.9 | NC_002484.1 | + | 27724 | 0.66 | 0.760324 |
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Target: 5'- cGAGCcgcGCGCgGGCAGaaGUUUGGCCc -3' miRNA: 3'- -CUCGu--CGCGgUCGUCaaCAAGUUGGc -5' |
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| 8804 | 5' | -53.9 | NC_002484.1 | + | 13492 | 0.7 | 0.517464 |
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Target: 5'- gGAGCGGUgauGCCGGCAGccgaagCAGCCGc -3' miRNA: 3'- -CUCGUCG---CGGUCGUCaacaa-GUUGGC- -5' |
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| 8804 | 5' | -53.9 | NC_002484.1 | + | 8351 | 1.1 | 0.001077 |
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Target: 5'- gGAGCAGCGCCAGCAGUUGUUCAACCGg -3' miRNA: 3'- -CUCGUCGCGGUCGUCAACAAGUUGGC- -5' |
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| 8804 | 5' | -53.9 | NC_002484.1 | + | 4670 | 0.66 | 0.749783 |
|
Target: 5'- uGGGCAGU--CAGCAGUUcgUCGACCu -3' miRNA: 3'- -CUCGUCGcgGUCGUCAAcaAGUUGGc -5' |
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| 8804 | 5' | -53.9 | NC_002484.1 | + | 3842 | 0.68 | 0.639498 |
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Target: 5'- gGAGCgcagAGCGCCAGCAaugcGUUUuGCCGu -3' miRNA: 3'- -CUCG----UCGCGGUCGUcaa-CAAGuUGGC- -5' |
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| 8804 | 5' | -53.9 | NC_002484.1 | + | 2186 | 0.68 | 0.616992 |
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Target: 5'- aGGUAGagGCCAGUAGcgaUGUUCAugCGa -3' miRNA: 3'- cUCGUCg-CGGUCGUCa--ACAAGUugGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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