Results 1 - 9 of 9 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
89 | 3' | -54.7 | AC_000006.1 | + | 17275 | 0.66 | 0.644246 |
Target: 5'- uGCGGcAUCauCGAGGGGGuggccauCUUCGCgCg -3' miRNA: 3'- -CGUC-UAGacGCUCCCCUu------GAAGCGgG- -5' |
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89 | 3' | -54.7 | AC_000006.1 | + | 12372 | 0.66 | 0.610172 |
Target: 5'- cGCGGG-CUGUGGGGcGuGCaggCGCCCg -3' miRNA: 3'- -CGUCUaGACGCUCCcCuUGaa-GCGGG- -5' |
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89 | 3' | -54.7 | AC_000006.1 | + | 3453 | 0.67 | 0.576278 |
Target: 5'- gGCGGGgcCUuCGaAGGGGGGCUUUuuaGCCCu -3' miRNA: 3'- -CGUCUa-GAcGC-UCCCCUUGAAG---CGGG- -5' |
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89 | 3' | -54.7 | AC_000006.1 | + | 12011 | 0.68 | 0.506797 |
Target: 5'- cGUAGGcccgacacagcuccUCgacGCuGAGGGGggUUUCGCuCCg -3' miRNA: 3'- -CGUCU--------------AGa--CG-CUCCCCuuGAAGCG-GG- -5' |
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89 | 3' | -54.7 | AC_000006.1 | + | 10331 | 0.68 | 0.496079 |
Target: 5'- uCGGca-UGCuGGGGGAgacccggguugagcACUUCGCCCg -3' miRNA: 3'- cGUCuagACGcUCCCCU--------------UGAAGCGGG- -5' |
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89 | 3' | -54.7 | AC_000006.1 | + | 13344 | 0.69 | 0.447183 |
Target: 5'- uGCAGcgCgGCGcGGGGAugauGCUgauGCCCg -3' miRNA: 3'- -CGUCuaGaCGCuCCCCU----UGAag-CGGG- -5' |
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89 | 3' | -54.7 | AC_000006.1 | + | 33768 | 0.74 | 0.220289 |
Target: 5'- gGUGGGUCUGUGcacuugcAGGGGcgUUUCGCCUg -3' miRNA: 3'- -CGUCUAGACGC-------UCCCCuuGAAGCGGG- -5' |
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89 | 3' | -54.7 | AC_000006.1 | + | 25014 | 0.85 | 0.036106 |
Target: 5'- uGCaAGA-CUGCGGGGGGAACaucguUUCGCCCg -3' miRNA: 3'- -CG-UCUaGACGCUCCCCUUG-----AAGCGGG- -5' |
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89 | 3' | -54.7 | AC_000006.1 | + | 30485 | 1.13 | 0.000358 |
Target: 5'- uGCAGAUCUGCGAGGGGAACUUCGCCCc -3' miRNA: 3'- -CGUCUAGACGCUCCCCUUGAAGCGGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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