Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 8924 | 5' | -44.5 | NC_002484.1 | + | 26042 | 0.66 | 0.998474 |
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Target: 5'- --cGCGACcgAUaaaGCGCGgGCGGAGAUc -3' miRNA: 3'- uauCGUUGuaUA---UGUGUgCGUCUUUA- -5' |
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| 8924 | 5' | -44.5 | NC_002484.1 | + | 44170 | 0.66 | 0.998113 |
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Target: 5'- -cAGUAGCAaGU-CGCGCGCAaGAGAg -3' miRNA: 3'- uaUCGUUGUaUAuGUGUGCGU-CUUUa -5' |
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| 8924 | 5' | -44.5 | NC_002484.1 | + | 43038 | 0.67 | 0.995875 |
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Target: 5'- --cGCAGCAggcUACGCA-GCAGAAGc -3' miRNA: 3'- uauCGUUGUau-AUGUGUgCGUCUUUa -5' |
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| 8924 | 5' | -44.5 | NC_002484.1 | + | 7939 | 0.67 | 0.995063 |
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Target: 5'- uUGGguGCAUGaACGCACGCAu---- -3' miRNA: 3'- uAUCguUGUAUaUGUGUGCGUcuuua -5' |
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| 8924 | 5' | -44.5 | NC_002484.1 | + | 40510 | 0.67 | 0.994126 |
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Target: 5'- gAUGGCugccGCAUGUGCGCGagacgguaGCGGAAc- -3' miRNA: 3'- -UAUCGu---UGUAUAUGUGUg-------CGUCUUua -5' |
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| 8924 | 5' | -44.5 | NC_002484.1 | + | 4841 | 0.67 | 0.99305 |
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Target: 5'- --cGCGGCAUggAUACGCgaugcgagccGCGCAGGGAg -3' miRNA: 3'- uauCGUUGUA--UAUGUG----------UGCGUCUUUa -5' |
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| 8924 | 5' | -44.5 | NC_002484.1 | + | 37812 | 0.68 | 0.990428 |
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Target: 5'- -aGGCAAUcguucuGUGUGC-CGCGCAGAu-- -3' miRNA: 3'- uaUCGUUG------UAUAUGuGUGCGUCUuua -5' |
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| 8924 | 5' | -44.5 | NC_002484.1 | + | 14114 | 0.7 | 0.964299 |
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Target: 5'- cUGGCGGCA--UACGCACGCAa---- -3' miRNA: 3'- uAUCGUUGUauAUGUGUGCGUcuuua -5' |
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| 8924 | 5' | -44.5 | NC_002484.1 | + | 30046 | 0.71 | 0.944853 |
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Target: 5'- gGUAGCAACuggacuucgagGUACGCACGCAuGAc-- -3' miRNA: 3'- -UAUCGUUGua---------UAUGUGUGCGU-CUuua -5' |
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| 8924 | 5' | -44.5 | NC_002484.1 | + | 15333 | 0.73 | 0.893018 |
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Target: 5'- cUGGCGGgAUAUGC-CAUGCAGAAu- -3' miRNA: 3'- uAUCGUUgUAUAUGuGUGCGUCUUua -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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