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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 8934 | 3' | -54.8 | NC_002484.1 | + | 51818 | 0.7 | 0.47879 |
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Target: 5'- uGUCCUUGaugGCCAAgGCGcAGGCCgGg -3' miRNA: 3'- -CGGGAACga-UGGUUgUGCuUCCGGgU- -5' |
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| 8934 | 3' | -54.8 | NC_002484.1 | + | 48885 | 0.7 | 0.458499 |
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Target: 5'- cCCCUUGCccucuCCuucCACG-AGGCCCAg -3' miRNA: 3'- cGGGAACGau---GGuu-GUGCuUCCGGGU- -5' |
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| 8934 | 3' | -54.8 | NC_002484.1 | + | 37884 | 0.66 | 0.707292 |
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Target: 5'- uGCCggcaggcgGCUGCCAGCAUGAGGuGCaCUg -3' miRNA: 3'- -CGGgaa-----CGAUGGUUGUGCUUC-CG-GGu -5' |
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| 8934 | 3' | -54.8 | NC_002484.1 | + | 36927 | 0.66 | 0.728847 |
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Target: 5'- aGCgCCUUGCcGCCGA-ACGccGaGCCCAg -3' miRNA: 3'- -CG-GGAACGaUGGUUgUGCuuC-CGGGU- -5' |
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| 8934 | 3' | -54.8 | NC_002484.1 | + | 32448 | 0.74 | 0.307264 |
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Target: 5'- cGCCCUU-CUGCgAGagaACGAAGGCCUg -3' miRNA: 3'- -CGGGAAcGAUGgUUg--UGCUUCCGGGu -5' |
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| 8934 | 3' | -54.8 | NC_002484.1 | + | 27734 | 0.67 | 0.652294 |
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Target: 5'- gGCCCUgUGCcgaGCCGcGCGCGGGcagaaguuuGGCCCGa -3' miRNA: 3'- -CGGGA-ACGa--UGGU-UGUGCUU---------CCGGGU- -5' |
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| 8934 | 3' | -54.8 | NC_002484.1 | + | 12226 | 0.66 | 0.728847 |
|
Target: 5'- gGCaaucgaCUUGcCUACaAGCugGAAGGCuCCAa -3' miRNA: 3'- -CGg-----GAAC-GAUGgUUGugCUUCCG-GGU- -5' |
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| 8934 | 3' | -54.8 | NC_002484.1 | + | 8318 | 0.73 | 0.314235 |
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Target: 5'- cGCCCUUGUggaacuggaugucUGCCGGCGgGAGuggaucgauGGCCCAu -3' miRNA: 3'- -CGGGAACG-------------AUGGUUGUgCUU---------CCGGGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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