Results 1 - 15 of 15 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
8948 | 3' | -55.6 | NC_002512.2 | + | 220997 | 1.08 | 0.005442 |
Target: 5'- gGUCGAGUGCCCCAACGGCUUCUGUCAc -3' miRNA: 3'- -CAGCUCACGGGGUUGCCGAAGACAGU- -5' |
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8948 | 3' | -55.6 | NC_002512.2 | + | 187281 | 0.69 | 0.85644 |
Target: 5'- cUCGaAGUGCCCCcg-GGCUUCggGUCc -3' miRNA: 3'- cAGC-UCACGGGGuugCCGAAGa-CAGu -5' |
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8948 | 3' | -55.6 | NC_002512.2 | + | 174574 | 0.66 | 0.950471 |
Target: 5'- aGUCGAGUGCCggGACGaa-UCUGUCu -3' miRNA: 3'- -CAGCUCACGGggUUGCcgaAGACAGu -5' |
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8948 | 3' | -55.6 | NC_002512.2 | + | 167563 | 0.68 | 0.898404 |
Target: 5'- --gGGGUGCCCgu-CGGCUUCUucccgGUCAu -3' miRNA: 3'- cagCUCACGGGguuGCCGAAGA-----CAGU- -5' |
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8948 | 3' | -55.6 | NC_002512.2 | + | 139819 | 0.73 | 0.684943 |
Target: 5'- gGUCGGGUGUCCaaggaCGugGGCgagUUCUGUCGc -3' miRNA: 3'- -CAGCUCACGGG-----GUugCCG---AAGACAGU- -5' |
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8948 | 3' | -55.6 | NC_002512.2 | + | 124262 | 0.67 | 0.937257 |
Target: 5'- -aCG-GUGCaCCCGACGGCggC-GUCGg -3' miRNA: 3'- caGCuCACG-GGGUUGCCGaaGaCAGU- -5' |
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8948 | 3' | -55.6 | NC_002512.2 | + | 117124 | 0.66 | 0.946293 |
Target: 5'- -aCGGG-GCCCCGucgGCGGCgUUCgaGUCGc -3' miRNA: 3'- caGCUCaCGGGGU---UGCCG-AAGa-CAGU- -5' |
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8948 | 3' | -55.6 | NC_002512.2 | + | 111676 | 0.66 | 0.954427 |
Target: 5'- -aCGAGgGCCCCGucgcaccagACGGCcUCgggGUCGc -3' miRNA: 3'- caGCUCaCGGGGU---------UGCCGaAGa--CAGU- -5' |
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8948 | 3' | -55.6 | NC_002512.2 | + | 99233 | 0.69 | 0.837619 |
Target: 5'- gGUCGugcgcaucuGGUGCCCCGGCGccgucaccgagucCUUCUGUCGc -3' miRNA: 3'- -CAGC---------UCACGGGGUUGCc------------GAAGACAGU- -5' |
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8948 | 3' | -55.6 | NC_002512.2 | + | 97801 | 0.66 | 0.946293 |
Target: 5'- gGUCGAGcGCUCCGGCuccgucgacgGGCUcCUGUUg -3' miRNA: 3'- -CAGCUCaCGGGGUUG----------CCGAaGACAGu -5' |
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8948 | 3' | -55.6 | NC_002512.2 | + | 82032 | 0.66 | 0.954427 |
Target: 5'- gGUCGGGgucGCgCCGGCGGCg---GUCGc -3' miRNA: 3'- -CAGCUCa--CGgGGUUGCCGaagaCAGU- -5' |
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8948 | 3' | -55.6 | NC_002512.2 | + | 48558 | 0.7 | 0.807475 |
Target: 5'- -cUGA-UGCCCCGGCGGCUggagCUGUa- -3' miRNA: 3'- caGCUcACGGGGUUGCCGAa---GACAgu -5' |
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8948 | 3' | -55.6 | NC_002512.2 | + | 37324 | 0.68 | 0.910649 |
Target: 5'- -cCGAG-GCCCCGgccACGGaCgcgCUGUCGg -3' miRNA: 3'- caGCUCaCGGGGU---UGCC-Gaa-GACAGU- -5' |
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8948 | 3' | -55.6 | NC_002512.2 | + | 34054 | 0.69 | 0.840831 |
Target: 5'- cUCGAggcGUGCCCCAcgACGGCggC-GUCGa -3' miRNA: 3'- cAGCU---CACGGGGU--UGCCGaaGaCAGU- -5' |
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8948 | 3' | -55.6 | NC_002512.2 | + | 19705 | 0.66 | 0.950471 |
Target: 5'- gGUCGggacgcAGUGCUCCuggaugAACGGCccgCUGUCGa -3' miRNA: 3'- -CAGC------UCACGGGG------UUGCCGaa-GACAGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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