miRNA: 3'- gCGACCGCCGCUGucaCGAuaGCAGc -5'
miRNA: 3'- -GCGaCCGCCGCUGUCa-----CGa---UAGCAGC- -5'
miRNA: 3'- -GCGaCCGCCGCUGUCa-CGa-UAGCAGc -5'
miRNA: 3'- -GCGACCGCCGCUGUCACGAUAGCAGC- -5'
Results 61 - 64 of 64 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 8955 | 3' | -56.8 | NC_002512.2 | + | 26700 | 0.75 | 0.518337 |
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Target: 5'- uGCUGGCGGUGACcugGCUggCGUCc -3' miRNA: 3'- gCGACCGCCGCUGucaCGAuaGCAGc -5' |
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| 8955 | 3' | -56.8 | NC_002512.2 | + | 83816 | 0.76 | 0.454176 |
|
Target: 5'- gCGCcGGCGGCGGCGGcuccggGCccggGUCGUCGc -3' miRNA: 3'- -GCGaCCGCCGCUGUCa-----CGa---UAGCAGC- -5' |
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| 8955 | 3' | -56.8 | NC_002512.2 | + | 188648 | 0.76 | 0.44536 |
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Target: 5'- gCGCcGGCGGCGGCGGcgGCccGUCGUCc -3' miRNA: 3'- -GCGaCCGCCGCUGUCa-CGa-UAGCAGc -5' |
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| 8955 | 3' | -56.8 | NC_002512.2 | + | 213370 | 1.1 | 0.003698 |
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Target: 5'- gCGCUGGCGGCGACAGUGCUAUCGUCGg -3' miRNA: 3'- -GCGACCGCCGCUGUCACGAUAGCAGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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