miRNA: 3'- gCGA--CCGCCGCUG--------UCA--CGa--------UAGCAGC- -5'
miRNA: 3'- gCGaCCGCC-GCUGUCaCGAUag----CAGC- -5'
miRNA: 3'- gCGa--CCGCCGCUGu-----CA--CGaUAGCAGC- -5'
miRNA: 3'- -GCGaCCGCCGCUG-----------UCACG----A--------------UAGCAGC- -5'
Results 61 - 64 of 64 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
|
Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 8955 | 3' | -56.8 | NC_002512.2 | + | 188564 | 0.67 | 0.901219 |
|
Target: 5'- aGCUccGGCGGCGACgacuucucGGUcuGCgucgaccagGUCGUCGa -3' miRNA: 3'- gCGA--CCGCCGCUG--------UCA--CGa--------UAGCAGC- -5' |
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| 8955 | 3' | -56.8 | NC_002512.2 | + | 160837 | 0.67 | 0.894978 |
|
Target: 5'- gGCgGGCGGcCGGCGGcGCUGggcgcaGUCGc -3' miRNA: 3'- gCGaCCGCC-GCUGUCaCGAUag----CAGC- -5' |
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| 8955 | 3' | -56.8 | NC_002512.2 | + | 126449 | 0.67 | 0.894978 |
|
Target: 5'- gGCgacGGCGGCGGCcgccuuGUucGCcGUCGUCGu -3' miRNA: 3'- gCGa--CCGCCGCUGu-----CA--CGaUAGCAGC- -5' |
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| 8955 | 3' | -56.8 | NC_002512.2 | + | 113726 | 0.67 | 0.893064 |
|
Target: 5'- aCGCgGGCGGCGAUcugcgggccgaGGUGCgcggUcauggagccgaccaGUCGUCGa -3' miRNA: 3'- -GCGaCCGCCGCUG-----------UCACG----A--------------UAGCAGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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