Results 21 - 29 of 29 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 8976 | 3' | -55.2 | NC_002512.2 | + | 158221 | 0.69 | 0.86452 |
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Target: 5'- aCCUCcUGUccGGGCCGCCcaagUUCGGc -3' miRNA: 3'- aGGAGuACG--CCCGGCGGaua-AAGCUa -5' |
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| 8976 | 3' | -55.2 | NC_002512.2 | + | 162000 | 0.69 | 0.885262 |
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Target: 5'- aCCUgGUGCGGGaugcggaCCGCCUGgc-CGAc -3' miRNA: 3'- aGGAgUACGCCC-------GGCGGAUaaaGCUa -5' |
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| 8976 | 3' | -55.2 | NC_002512.2 | + | 167426 | 0.66 | 0.955204 |
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Target: 5'- aUCUCGUGCGgcGGCaCGCCUGcgcgUUCGu- -3' miRNA: 3'- aGGAGUACGC--CCG-GCGGAUa---AAGCua -5' |
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| 8976 | 3' | -55.2 | NC_002512.2 | + | 183485 | 0.71 | 0.771571 |
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Target: 5'- gCCgugC-UGCGGGCCGCCUAcggccgCGAg -3' miRNA: 3'- aGGa--GuACGCCCGGCGGAUaaa---GCUa -5' |
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| 8976 | 3' | -55.2 | NC_002512.2 | + | 197189 | 0.69 | 0.871874 |
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Target: 5'- gUCCUCAUGgacccgcucgGGGCCGUCUAcggcUUCGAc -3' miRNA: 3'- -AGGAGUACg---------CCCGGCGGAUa---AAGCUa -5' |
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| 8976 | 3' | -55.2 | NC_002512.2 | + | 197611 | 1.05 | 0.007856 |
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Target: 5'- cUCCUCAUGCGGGCCGCCUAUUUCGAUc -3' miRNA: 3'- -AGGAGUACGCCCGGCGGAUAAAGCUA- -5' |
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| 8976 | 3' | -55.2 | NC_002512.2 | + | 204021 | 0.74 | 0.607332 |
|
Target: 5'- gCUUCGUGCGGGCCGacggcgaacucucgcCCUAcUUCGGUc -3' miRNA: 3'- aGGAGUACGCCCGGC---------------GGAUaAAGCUA- -5' |
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| 8976 | 3' | -55.2 | NC_002512.2 | + | 214629 | 0.66 | 0.955204 |
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Target: 5'- cCCUCGcggggacGCGGGCCGCCUcgcagUCu-- -3' miRNA: 3'- aGGAGUa------CGCCCGGCGGAuaa--AGcua -5' |
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| 8976 | 3' | -55.2 | NC_002512.2 | + | 218217 | 0.66 | 0.965716 |
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Target: 5'- gCCUCGggccgcgGCGGGCCGCggcgc-CGAUa -3' miRNA: 3'- aGGAGUa------CGCCCGGCGgauaaaGCUA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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