Results 21 - 29 of 29 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 8981 | 3' | -52.2 | NC_002512.2 | + | 4317 | 0.71 | 0.923484 |
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Target: 5'- gCCCCUCGAGGUCGGCCucGUcGCc-GAAg -3' miRNA: 3'- -GGGGAGUUCCAGUUGG--CGaUGuaCUU- -5' |
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| 8981 | 3' | -52.2 | NC_002512.2 | + | 139459 | 0.71 | 0.905878 |
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Target: 5'- aCCgUCGA-GUCGACCGCUACuAUGGc -3' miRNA: 3'- gGGgAGUUcCAGUUGGCGAUG-UACUu -5' |
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| 8981 | 3' | -52.2 | NC_002512.2 | + | 213154 | 0.71 | 0.905878 |
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Target: 5'- aCCCgCUCAuggcGGGUCAGCU-CUGCGUGGu -3' miRNA: 3'- -GGG-GAGU----UCCAGUUGGcGAUGUACUu -5' |
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| 8981 | 3' | -52.2 | NC_002512.2 | + | 98614 | 0.71 | 0.899531 |
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Target: 5'- gCCgUCgAAGGUCAAcCCGCUGC-UGGAg -3' miRNA: 3'- gGGgAG-UUCCAGUU-GGCGAUGuACUU- -5' |
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| 8981 | 3' | -52.2 | NC_002512.2 | + | 60335 | 0.73 | 0.840661 |
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Target: 5'- aCCCUCGuAGGUCAGCCcccucccaUACAUGAc -3' miRNA: 3'- gGGGAGU-UCCAGUUGGcg------AUGUACUu -5' |
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| 8981 | 3' | -52.2 | NC_002512.2 | + | 186934 | 0.73 | 0.840661 |
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Target: 5'- gCCCgu--GGUCAACCGCUACcUGGc -3' miRNA: 3'- gGGGaguuCCAGUUGGCGAUGuACUu -5' |
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| 8981 | 3' | -52.2 | NC_002512.2 | + | 144602 | 0.73 | 0.823896 |
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Target: 5'- uCCCCUCGcacgAGGUCcgcGCCGCgcuggACGUGGc -3' miRNA: 3'- -GGGGAGU----UCCAGu--UGGCGa----UGUACUu -5' |
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| 8981 | 3' | -52.2 | NC_002512.2 | + | 225847 | 0.75 | 0.740708 |
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Target: 5'- cCCCUUCucGGGUgGACCGUagUACAUGAAc -3' miRNA: 3'- -GGGGAGu-UCCAgUUGGCG--AUGUACUU- -5' |
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| 8981 | 3' | -52.2 | NC_002512.2 | + | 194577 | 1.09 | 0.009997 |
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Target: 5'- gCCCCUCAAGGUCAACCGCUACAUGAAg -3' miRNA: 3'- -GGGGAGUUCCAGUUGGCGAUGUACUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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