Results 1 - 20 of 43 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
8992 | 3' | -58.2 | NC_002512.2 | + | 194310 | 0.66 | 0.903301 |
Target: 5'- aCAGGUCAccuUCCucguggcCCGGGACGGUuGCGu -3' miRNA: 3'- -GUCUAGUac-GGGu------GGCCCUGCCA-CGU- -5' |
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8992 | 3' | -58.2 | NC_002512.2 | + | 7341 | 0.66 | 0.903301 |
Target: 5'- uCGGGUCGccguagccccgGCCCACCGcGACGGcgGCc -3' miRNA: 3'- -GUCUAGUa----------CGGGUGGCcCUGCCa-CGu -5' |
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8992 | 3' | -58.2 | NC_002512.2 | + | 108546 | 0.66 | 0.903301 |
Target: 5'- gCGGAgaagGUCCGCCGGGccuuccgacCGGUGCu -3' miRNA: 3'- -GUCUaguaCGGGUGGCCCu--------GCCACGu -5' |
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8992 | 3' | -58.2 | NC_002512.2 | + | 11284 | 0.66 | 0.903301 |
Target: 5'- --cAUgGUGCCCACagCGGGACGaGUuGCGa -3' miRNA: 3'- gucUAgUACGGGUG--GCCCUGC-CA-CGU- -5' |
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8992 | 3' | -58.2 | NC_002512.2 | + | 72796 | 0.66 | 0.903301 |
Target: 5'- cCGGGaCggGCCCGCCGuGGGCGccgGCGa -3' miRNA: 3'- -GUCUaGuaCGGGUGGC-CCUGCca-CGU- -5' |
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8992 | 3' | -58.2 | NC_002512.2 | + | 131949 | 0.66 | 0.903301 |
Target: 5'- gUAGAaccUCAUGCCCGgCGGaucGCGGUagGCGa -3' miRNA: 3'- -GUCU---AGUACGGGUgGCCc--UGCCA--CGU- -5' |
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8992 | 3' | -58.2 | NC_002512.2 | + | 35041 | 0.66 | 0.902695 |
Target: 5'- -cGAUCGggGUCCGCaCGGGccgggacagcaccACGGUGCc -3' miRNA: 3'- guCUAGUa-CGGGUG-GCCC-------------UGCCACGu -5' |
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8992 | 3' | -58.2 | NC_002512.2 | + | 153349 | 0.66 | 0.897143 |
Target: 5'- cCGGGUCGgcggGuCCCGCUGGGcUGGgcgGCGg -3' miRNA: 3'- -GUCUAGUa---C-GGGUGGCCCuGCCa--CGU- -5' |
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8992 | 3' | -58.2 | NC_002512.2 | + | 119016 | 0.66 | 0.897143 |
Target: 5'- uGGAUCGUGCcgcugCCGCUGGcGACGGa--- -3' miRNA: 3'- gUCUAGUACG-----GGUGGCC-CUGCCacgu -5' |
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8992 | 3' | -58.2 | NC_002512.2 | + | 219844 | 0.66 | 0.897143 |
Target: 5'- gGGGUCcgGCCCG--GGGGCGGaucGCGg -3' miRNA: 3'- gUCUAGuaCGGGUggCCCUGCCa--CGU- -5' |
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8992 | 3' | -58.2 | NC_002512.2 | + | 123993 | 0.66 | 0.89077 |
Target: 5'- -cGAcggCGaGCCCACCGGGugGGa--- -3' miRNA: 3'- guCUa--GUaCGGGUGGCCCugCCacgu -5' |
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8992 | 3' | -58.2 | NC_002512.2 | + | 119423 | 0.66 | 0.884187 |
Target: 5'- gGGAgCGUGgaCCGCgGGGACcuGGUGCGc -3' miRNA: 3'- gUCUaGUACg-GGUGgCCCUG--CCACGU- -5' |
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8992 | 3' | -58.2 | NC_002512.2 | + | 133215 | 0.66 | 0.877397 |
Target: 5'- gGGAcgUCGUccGCCCAgCGaGGACGGgaggGCGg -3' miRNA: 3'- gUCU--AGUA--CGGGUgGC-CCUGCCa---CGU- -5' |
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8992 | 3' | -58.2 | NC_002512.2 | + | 75557 | 0.66 | 0.877397 |
Target: 5'- ----gCggGCUCGCCGGGGCGGgGCc -3' miRNA: 3'- gucuaGuaCGGGUGGCCCUGCCaCGu -5' |
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8992 | 3' | -58.2 | NC_002512.2 | + | 47678 | 0.67 | 0.870404 |
Target: 5'- -cGAaCGUGCuguuCCACCcgGGGGCGGUGUu -3' miRNA: 3'- guCUaGUACG----GGUGG--CCCUGCCACGu -5' |
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8992 | 3' | -58.2 | NC_002512.2 | + | 17834 | 0.67 | 0.863213 |
Target: 5'- cCAGggUGUGCgCCAgcagcuccucCCGGGcGCGGUGCu -3' miRNA: 3'- -GUCuaGUACG-GGU----------GGCCC-UGCCACGu -5' |
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8992 | 3' | -58.2 | NC_002512.2 | + | 83976 | 0.67 | 0.863213 |
Target: 5'- gCGGA---UGCCCgGCgCGGGGCGGUcGCGc -3' miRNA: 3'- -GUCUaguACGGG-UG-GCCCUGCCA-CGU- -5' |
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8992 | 3' | -58.2 | NC_002512.2 | + | 171849 | 0.67 | 0.855829 |
Target: 5'- -cGGUCcgGCCgCgGCCGGGAgGGgGCGg -3' miRNA: 3'- guCUAGuaCGG-G-UGGCCCUgCCaCGU- -5' |
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8992 | 3' | -58.2 | NC_002512.2 | + | 154435 | 0.67 | 0.848257 |
Target: 5'- gCGGAUCcuUGUCCggcGCCGucGACGGUGCGu -3' miRNA: 3'- -GUCUAGu-ACGGG---UGGCc-CUGCCACGU- -5' |
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8992 | 3' | -58.2 | NC_002512.2 | + | 219402 | 0.67 | 0.843627 |
Target: 5'- -uGGUCG-GCCCGCCgcgacgcccacgcccGGGGCGGcgGCGg -3' miRNA: 3'- guCUAGUaCGGGUGG---------------CCCUGCCa-CGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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