Results 21 - 25 of 25 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
90 | 5' | -60.4 | AC_000006.1 | + | 26962 | 0.73 | 0.105158 |
Target: 5'- -cCCUCGUcuucGCUCucCGCCCCCGCCg -3' miRNA: 3'- gaGGAGCGu---CGAGuaGUGGGGGCGGg -5' |
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90 | 5' | -60.4 | AC_000006.1 | + | 26361 | 0.74 | 0.088707 |
Target: 5'- cCUUCUCGCAGCUCAugggUCGCaucucgaaugaCCUCGCCg -3' miRNA: 3'- -GAGGAGCGUCGAGU----AGUG-----------GGGGCGGg -5' |
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90 | 5' | -60.4 | AC_000006.1 | + | 21679 | 0.74 | 0.085236 |
Target: 5'- --aCUCGCAGUgcacgggcaucagCAUCAUCCCCGCgCCg -3' miRNA: 3'- gagGAGCGUCGa------------GUAGUGGGGGCG-GG- -5' |
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90 | 5' | -60.4 | AC_000006.1 | + | 23460 | 0.75 | 0.081195 |
Target: 5'- aCUCCUCGCcGC-CGgcggCGCCCCCuaccagcGCCCg -3' miRNA: 3'- -GAGGAGCGuCGaGUa---GUGGGGG-------CGGG- -5' |
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90 | 5' | -60.4 | AC_000006.1 | + | 30389 | 1.1 | 0.000152 |
Target: 5'- uCUCCUCGCAGCUCAUCACCCCCGCCCc -3' miRNA: 3'- -GAGGAGCGUCGAGUAGUGGGGGCGGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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