Results 1 - 20 of 29 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
9035 | 5' | -56.4 | NC_002512.2 | + | 82488 | 0.66 | 0.964392 |
Target: 5'- gCCguuUgACGCCgguCCUCUGcaUGGCGUGGu -3' miRNA: 3'- aGGau-AgUGCGG---GGAGGC--ACUGCACC- -5' |
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9035 | 5' | -56.4 | NC_002512.2 | + | 116720 | 0.66 | 0.964392 |
Target: 5'- gCCgGUCGCGUCCCgcccggCCGcGGCGaGGc -3' miRNA: 3'- aGGaUAGUGCGGGGa-----GGCaCUGCaCC- -5' |
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9035 | 5' | -56.4 | NC_002512.2 | + | 125743 | 0.66 | 0.964392 |
Target: 5'- gUCCggGUCGCGUCCCccagcccgUCCGUGAUc--- -3' miRNA: 3'- -AGGa-UAGUGCGGGG--------AGGCACUGcacc -5' |
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9035 | 5' | -56.4 | NC_002512.2 | + | 217683 | 0.66 | 0.964074 |
Target: 5'- cUCUAUCAguuuucauauuuuCGCCCCUCUuauauGCGUGGu -3' miRNA: 3'- aGGAUAGU-------------GCGGGGAGGcac--UGCACC- -5' |
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9035 | 5' | -56.4 | NC_002512.2 | + | 97829 | 0.66 | 0.963111 |
Target: 5'- cUCCUGuuggacuguUCGCGCaCCCUCCGgucccuggccgacGACGUc- -3' miRNA: 3'- -AGGAU---------AGUGCG-GGGAGGCa------------CUGCAcc -5' |
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9035 | 5' | -56.4 | NC_002512.2 | + | 60259 | 0.66 | 0.961129 |
Target: 5'- aCCUAUCAgGCCCCUCUcauauagGACu--- -3' miRNA: 3'- aGGAUAGUgCGGGGAGGca-----CUGcacc -5' |
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9035 | 5' | -56.4 | NC_002512.2 | + | 165781 | 0.66 | 0.961129 |
Target: 5'- cUCCUG-CGCGCCCgUCgagGACGUcuGGg -3' miRNA: 3'- -AGGAUaGUGCGGGgAGgcaCUGCA--CC- -5' |
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9035 | 5' | -56.4 | NC_002512.2 | + | 205425 | 0.66 | 0.961129 |
Target: 5'- cUCCUAUgCuaagagGCGCCCCaCCGUG-CGUucGGa -3' miRNA: 3'- -AGGAUA-G------UGCGGGGaGGCACuGCA--CC- -5' |
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9035 | 5' | -56.4 | NC_002512.2 | + | 185449 | 0.66 | 0.961129 |
Target: 5'- gUCCUGUC-CGCCCucugCUCCuucGcCGUGGu -3' miRNA: 3'- -AGGAUAGuGCGGG----GAGGca-CuGCACC- -5' |
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9035 | 5' | -56.4 | NC_002512.2 | + | 139187 | 0.66 | 0.961129 |
Target: 5'- cCCUGUCACGCCgaCgCCGUcgagGACcUGGc -3' miRNA: 3'- aGGAUAGUGCGGg-GaGGCA----CUGcACC- -5' |
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9035 | 5' | -56.4 | NC_002512.2 | + | 171971 | 0.66 | 0.957666 |
Target: 5'- uUCCUcUUACGUCCCgCCuuagGAgGUGGg -3' miRNA: 3'- -AGGAuAGUGCGGGGaGGca--CUgCACC- -5' |
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9035 | 5' | -56.4 | NC_002512.2 | + | 193919 | 0.66 | 0.953997 |
Target: 5'- ---gAUCGCGUCCCggacUCCGcUGACGgccgGGg -3' miRNA: 3'- aggaUAGUGCGGGG----AGGC-ACUGCa---CC- -5' |
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9035 | 5' | -56.4 | NC_002512.2 | + | 47713 | 0.66 | 0.951695 |
Target: 5'- aUCCUG-CGCGCCgCCUCgCGggagGAgggcucggaacagauCGUGGa -3' miRNA: 3'- -AGGAUaGUGCGG-GGAG-GCa---CU---------------GCACC- -5' |
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9035 | 5' | -56.4 | NC_002512.2 | + | 206029 | 0.67 | 0.941725 |
Target: 5'- aCCUGauaCACGuCCCCuUCCGUcgUGUGGa -3' miRNA: 3'- aGGAUa--GUGC-GGGG-AGGCAcuGCACC- -5' |
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9035 | 5' | -56.4 | NC_002512.2 | + | 68385 | 0.67 | 0.932466 |
Target: 5'- gUCCgcgcaGCGCaccaggUCCUCCuUGACGUGGg -3' miRNA: 3'- -AGGauag-UGCG------GGGAGGcACUGCACC- -5' |
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9035 | 5' | -56.4 | NC_002512.2 | + | 83394 | 0.68 | 0.916385 |
Target: 5'- cCCg--CGCGUCCCcgCCGUGGggguuucCGUGGu -3' miRNA: 3'- aGGauaGUGCGGGGa-GGCACU-------GCACC- -5' |
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9035 | 5' | -56.4 | NC_002512.2 | + | 226410 | 0.68 | 0.911322 |
Target: 5'- aCCcGUCGCGUCCgcguCUCCG-GACGcGGg -3' miRNA: 3'- aGGaUAGUGCGGG----GAGGCaCUGCaCC- -5' |
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9035 | 5' | -56.4 | NC_002512.2 | + | 212010 | 0.68 | 0.905492 |
Target: 5'- gCCUAUCACGUCCC-CCG-GGCc--- -3' miRNA: 3'- aGGAUAGUGCGGGGaGGCaCUGcacc -5' |
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9035 | 5' | -56.4 | NC_002512.2 | + | 24790 | 0.68 | 0.899446 |
Target: 5'- gUCCgggCGCGCCCC-CCGagGACGg-- -3' miRNA: 3'- -AGGauaGUGCGGGGaGGCa-CUGCacc -5' |
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9035 | 5' | -56.4 | NC_002512.2 | + | 163129 | 0.68 | 0.892551 |
Target: 5'- cUCCUAUCACuucgucucccguaGCCucaccgccaCCUUCGUGACG-GGc -3' miRNA: 3'- -AGGAUAGUG-------------CGG---------GGAGGCACUGCaCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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