Results 21 - 29 of 29 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 9035 | 5' | -56.4 | NC_002512.2 | + | 137163 | 0.69 | 0.880047 |
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Target: 5'- aCCgagCGCGCCCC-CCG-GACGUc- -3' miRNA: 3'- aGGauaGUGCGGGGaGGCaCUGCAcc -5' |
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| 9035 | 5' | -56.4 | NC_002512.2 | + | 134979 | 0.69 | 0.873171 |
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Target: 5'- cUCCaUGUgCuCGCCCCgagagCUGcUGACGUGGa -3' miRNA: 3'- -AGG-AUA-GuGCGGGGa----GGC-ACUGCACC- -5' |
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| 9035 | 5' | -56.4 | NC_002512.2 | + | 221060 | 0.7 | 0.819799 |
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Target: 5'- gCCcGUCugGCCCaagCUCCGgcggcggaacGGCGUGGg -3' miRNA: 3'- aGGaUAGugCGGG---GAGGCa---------CUGCACC- -5' |
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| 9035 | 5' | -56.4 | NC_002512.2 | + | 10076 | 0.71 | 0.794452 |
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Target: 5'- cCCUGUCGCGCCCCgcgcguccccuaUCUGUGucccCGgccGGg -3' miRNA: 3'- aGGAUAGUGCGGGG------------AGGCACu---GCa--CC- -5' |
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| 9035 | 5' | -56.4 | NC_002512.2 | + | 137910 | 0.71 | 0.794452 |
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Target: 5'- cCCggaCGCGgCCCUCCG-GACGUGc -3' miRNA: 3'- aGGauaGUGCgGGGAGGCaCUGCACc -5' |
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| 9035 | 5' | -56.4 | NC_002512.2 | + | 44663 | 0.71 | 0.749628 |
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Target: 5'- aUUUAcUUACGCCC--CCGUGACGUGGa -3' miRNA: 3'- aGGAU-AGUGCGGGgaGGCACUGCACC- -5' |
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| 9035 | 5' | -56.4 | NC_002512.2 | + | 133711 | 0.72 | 0.73098 |
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Target: 5'- gCCUGuUCACGCUCaaCUUCGUGACGaGGa -3' miRNA: 3'- aGGAU-AGUGCGGG--GAGGCACUGCaCC- -5' |
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| 9035 | 5' | -56.4 | NC_002512.2 | + | 44585 | 0.74 | 0.604934 |
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Target: 5'- cUCC--UCGCGCCCCUCCaG-GACGuUGGc -3' miRNA: 3'- -AGGauAGUGCGGGGAGG-CaCUGC-ACC- -5' |
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| 9035 | 5' | -56.4 | NC_002512.2 | + | 151136 | 1.1 | 0.004157 |
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Target: 5'- cUCCUAUCACGCCCCUCCGUGACGUGGu -3' miRNA: 3'- -AGGAUAGUGCGGGGAGGCACUGCACC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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