Results 21 - 29 of 29 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 9035 | 5' | -56.4 | NC_002512.2 | + | 139187 | 0.66 | 0.961129 |
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Target: 5'- cCCUGUCACGCCgaCgCCGUcgagGACcUGGc -3' miRNA: 3'- aGGAUAGUGCGGg-GaGGCA----CUGcACC- -5' |
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| 9035 | 5' | -56.4 | NC_002512.2 | + | 185449 | 0.66 | 0.961129 |
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Target: 5'- gUCCUGUC-CGCCCucugCUCCuucGcCGUGGu -3' miRNA: 3'- -AGGAUAGuGCGGG----GAGGca-CuGCACC- -5' |
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| 9035 | 5' | -56.4 | NC_002512.2 | + | 205425 | 0.66 | 0.961129 |
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Target: 5'- cUCCUAUgCuaagagGCGCCCCaCCGUG-CGUucGGa -3' miRNA: 3'- -AGGAUA-G------UGCGGGGaGGCACuGCA--CC- -5' |
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| 9035 | 5' | -56.4 | NC_002512.2 | + | 165781 | 0.66 | 0.961129 |
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Target: 5'- cUCCUG-CGCGCCCgUCgagGACGUcuGGg -3' miRNA: 3'- -AGGAUaGUGCGGGgAGgcaCUGCA--CC- -5' |
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| 9035 | 5' | -56.4 | NC_002512.2 | + | 97829 | 0.66 | 0.963111 |
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Target: 5'- cUCCUGuuggacuguUCGCGCaCCCUCCGgucccuggccgacGACGUc- -3' miRNA: 3'- -AGGAU---------AGUGCG-GGGAGGCa------------CUGCAcc -5' |
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| 9035 | 5' | -56.4 | NC_002512.2 | + | 217683 | 0.66 | 0.964074 |
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Target: 5'- cUCUAUCAguuuucauauuuuCGCCCCUCUuauauGCGUGGu -3' miRNA: 3'- aGGAUAGU-------------GCGGGGAGGcac--UGCACC- -5' |
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| 9035 | 5' | -56.4 | NC_002512.2 | + | 125743 | 0.66 | 0.964392 |
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Target: 5'- gUCCggGUCGCGUCCCccagcccgUCCGUGAUc--- -3' miRNA: 3'- -AGGa-UAGUGCGGGG--------AGGCACUGcacc -5' |
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| 9035 | 5' | -56.4 | NC_002512.2 | + | 116720 | 0.66 | 0.964392 |
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Target: 5'- gCCgGUCGCGUCCCgcccggCCGcGGCGaGGc -3' miRNA: 3'- aGGaUAGUGCGGGGa-----GGCaCUGCaCC- -5' |
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| 9035 | 5' | -56.4 | NC_002512.2 | + | 82488 | 0.66 | 0.964392 |
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Target: 5'- gCCguuUgACGCCgguCCUCUGcaUGGCGUGGu -3' miRNA: 3'- aGGau-AgUGCGG---GGAGGC--ACUGCACC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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