Results 21 - 29 of 29 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 9055 | 3' | -52.8 | NC_002512.2 | + | 167902 | 0.68 | 0.981177 |
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Target: 5'- aGGAUCCguuUGAGGUugguuguugggUCUCGGAGUcgUGUCu -3' miRNA: 3'- -UCUGGG---GCUCCA-----------AGAGCUUCA--ACAGc -5' |
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| 9055 | 3' | -52.8 | NC_002512.2 | + | 174269 | 0.67 | 0.986611 |
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Target: 5'- cGGGUCCCGGGG-UCUCGggGgu-UCGc -3' miRNA: 3'- -UCUGGGGCUCCaAGAGCuuCaacAGC- -5' |
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| 9055 | 3' | -52.8 | NC_002512.2 | + | 184212 | 0.67 | 0.988123 |
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Target: 5'- cGGACCgCG-GGUUCUCucuGggGUCGa -3' miRNA: 3'- -UCUGGgGCuCCAAGAGcuuCaaCAGC- -5' |
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| 9055 | 3' | -52.8 | NC_002512.2 | + | 185416 | 0.68 | 0.971562 |
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Target: 5'- cGGCCCCGGGGcgccCUgGAGGUcuUCGa -3' miRNA: 3'- uCUGGGGCUCCaa--GAgCUUCAacAGC- -5' |
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| 9055 | 3' | -52.8 | NC_002512.2 | + | 192497 | 0.66 | 0.995351 |
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Target: 5'- gGGACgcgCUCGAGGUcuUCUCGGAGgacaugggGUCc -3' miRNA: 3'- -UCUG---GGGCUCCA--AGAGCUUCaa------CAGc -5' |
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| 9055 | 3' | -52.8 | NC_002512.2 | + | 199500 | 0.73 | 0.849654 |
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Target: 5'- aGGGCCgCGAGGaUCaggUCGAGGUcGUCGg -3' miRNA: 3'- -UCUGGgGCUCCaAG---AGCUUCAaCAGC- -5' |
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| 9055 | 3' | -52.8 | NC_002512.2 | + | 210949 | 0.66 | 0.994625 |
|
Target: 5'- -cGCCCCGAGGccaaCGAGGUcGUCc -3' miRNA: 3'- ucUGGGGCUCCaagaGCUUCAaCAGc -5' |
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| 9055 | 3' | -52.8 | NC_002512.2 | + | 213011 | 0.69 | 0.958837 |
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Target: 5'- cGGgCCCGGGGcccUCUCGAGGUgcGUCc -3' miRNA: 3'- uCUgGGGCUCCa--AGAGCUUCAa-CAGc -5' |
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| 9055 | 3' | -52.8 | NC_002512.2 | + | 221976 | 0.66 | 0.995351 |
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Target: 5'- aAGA-CCCGAGGUUCgUCcGGGUcGUCu -3' miRNA: 3'- -UCUgGGGCUCCAAG-AGcUUCAaCAGc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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