Results 21 - 29 of 29 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 9055 | 3' | -52.8 | NC_002512.2 | + | 71240 | 0.66 | 0.991879 |
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Target: 5'- uGGCCUCGAcgucGGgg-UCGAAGggGUCGg -3' miRNA: 3'- uCUGGGGCU----CCaagAGCUUCaaCAGC- -5' |
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| 9055 | 3' | -52.8 | NC_002512.2 | + | 132241 | 0.66 | 0.991879 |
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Target: 5'- gAGACggCGAGGUUCUCGAGcugcGUCa -3' miRNA: 3'- -UCUGggGCUCCAAGAGCUUcaa-CAGc -5' |
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| 9055 | 3' | -52.8 | NC_002512.2 | + | 125075 | 0.66 | 0.991879 |
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Target: 5'- uGGACCCCGccc-UgUCGAGGUUGaUCGu -3' miRNA: 3'- -UCUGGGGCuccaAgAGCUUCAAC-AGC- -5' |
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| 9055 | 3' | -52.8 | NC_002512.2 | + | 155380 | 0.66 | 0.994625 |
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Target: 5'- gGGACCgCGAGGcuggCGAGGUccucuccguccUGUCGc -3' miRNA: 3'- -UCUGGgGCUCCaagaGCUUCA-----------ACAGC- -5' |
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| 9055 | 3' | -52.8 | NC_002512.2 | + | 210949 | 0.66 | 0.994625 |
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Target: 5'- -cGCCCCGAGGccaaCGAGGUcGUCc -3' miRNA: 3'- ucUGGGGCUCCaagaGCUUCAaCAGc -5' |
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| 9055 | 3' | -52.8 | NC_002512.2 | + | 221976 | 0.66 | 0.995351 |
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Target: 5'- aAGA-CCCGAGGUUCgUCcGGGUcGUCu -3' miRNA: 3'- -UCUgGGGCUCCAAG-AGcUUCAaCAGc -5' |
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| 9055 | 3' | -52.8 | NC_002512.2 | + | 192497 | 0.66 | 0.995351 |
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Target: 5'- gGGACgcgCUCGAGGUcuUCUCGGAGgacaugggGUCc -3' miRNA: 3'- -UCUG---GGGCUCCA--AGAGCUUCaa------CAGc -5' |
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| 9055 | 3' | -52.8 | NC_002512.2 | + | 87543 | 0.66 | 0.995351 |
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Target: 5'- cGACCCUGAGGUcCagGAGGgcgGUUu -3' miRNA: 3'- uCUGGGGCUCCAaGagCUUCaa-CAGc -5' |
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| 9055 | 3' | -52.8 | NC_002512.2 | + | 128051 | 0.66 | 0.995351 |
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Target: 5'- cGGCCCCGAcGGUcg-CGgcGUcGUCGu -3' miRNA: 3'- uCUGGGGCU-CCAagaGCuuCAaCAGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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