Results 21 - 29 of 29 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 9055 | 3' | -52.8 | NC_002512.2 | + | 185416 | 0.68 | 0.971562 |
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Target: 5'- cGGCCCCGGGGcgccCUgGAGGUcuUCGa -3' miRNA: 3'- uCUGGGGCUCCaa--GAgCUUCAacAGC- -5' |
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| 9055 | 3' | -52.8 | NC_002512.2 | + | 35676 | 0.69 | 0.965612 |
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Target: 5'- gAGACagaCCGuGGUUUuuguUCGGAGUaGUCGg -3' miRNA: 3'- -UCUGg--GGCuCCAAG----AGCUUCAaCAGC- -5' |
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| 9055 | 3' | -52.8 | NC_002512.2 | + | 213011 | 0.69 | 0.958837 |
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Target: 5'- cGGgCCCGGGGcccUCUCGAGGUgcGUCc -3' miRNA: 3'- uCUgGGGCUCCa--AGAGCUUCAa-CAGc -5' |
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| 9055 | 3' | -52.8 | NC_002512.2 | + | 10230 | 0.7 | 0.937581 |
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Target: 5'- cAGAUCCCGAcgggcgaaccuccGGUUCUCGAAGaag-CGg -3' miRNA: 3'- -UCUGGGGCU-------------CCAAGAGCUUCaacaGC- -5' |
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| 9055 | 3' | -52.8 | NC_002512.2 | + | 135232 | 0.71 | 0.922839 |
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Target: 5'- cGGCCCCGGGccgcCUCGAGGccGUCGu -3' miRNA: 3'- uCUGGGGCUCcaa-GAGCUUCaaCAGC- -5' |
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| 9055 | 3' | -52.8 | NC_002512.2 | + | 199500 | 0.73 | 0.849654 |
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Target: 5'- aGGGCCgCGAGGaUCaggUCGAGGUcGUCGg -3' miRNA: 3'- -UCUGGgGCUCCaAG---AGCUUCAaCAGC- -5' |
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| 9055 | 3' | -52.8 | NC_002512.2 | + | 65686 | 0.73 | 0.825389 |
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Target: 5'- cGGCCCCGAGGccCUgcaUGAAGUcGUCGa -3' miRNA: 3'- uCUGGGGCUCCaaGA---GCUUCAaCAGC- -5' |
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| 9055 | 3' | -52.8 | NC_002512.2 | + | 3588 | 0.75 | 0.744249 |
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Target: 5'- cAGGCCCCGGGGgUCUCGggGa----- -3' miRNA: 3'- -UCUGGGGCUCCaAGAGCuuCaacagc -5' |
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| 9055 | 3' | -52.8 | NC_002512.2 | + | 144153 | 1.09 | 0.010584 |
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Target: 5'- gAGACCCCGAGGUUCUCGAAGUUGUCGc -3' miRNA: 3'- -UCUGGGGCUCCAAGAGCUUCAACAGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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