Results 1 - 20 of 85 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
9074 | 5' | -58.9 | NC_002512.2 | + | 3614 | 0.66 | 0.884436 |
Target: 5'- uCACGCGCCCcaaccUCCuACGGauuCUGUUCg- -3' miRNA: 3'- -GUGCGCGGGc----AGGuUGCC---GGCAAGaa -5' |
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9074 | 5' | -58.9 | NC_002512.2 | + | 134061 | 0.66 | 0.884436 |
Target: 5'- uGCGCGUCCGgugcagCCAucacCGGCUGcgCUg -3' miRNA: 3'- gUGCGCGGGCa-----GGUu---GCCGGCaaGAa -5' |
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9074 | 5' | -58.9 | NC_002512.2 | + | 79480 | 0.66 | 0.884436 |
Target: 5'- cCGCGCGCCCGUg----GGCgGUUCg- -3' miRNA: 3'- -GUGCGCGGGCAgguugCCGgCAAGaa -5' |
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9074 | 5' | -58.9 | NC_002512.2 | + | 79156 | 0.66 | 0.884436 |
Target: 5'- gCGCGgGCCgacgggaaGUCCAACGGUCGg---- -3' miRNA: 3'- -GUGCgCGGg-------CAGGUUGCCGGCaagaa -5' |
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9074 | 5' | -58.9 | NC_002512.2 | + | 95994 | 0.66 | 0.884436 |
Target: 5'- --aGCGCCCGggaCCGcCGGCCGa---- -3' miRNA: 3'- gugCGCGGGCa--GGUuGCCGGCaagaa -5' |
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9074 | 5' | -58.9 | NC_002512.2 | + | 115867 | 0.66 | 0.880409 |
Target: 5'- -cCGCGCCCGgugccgcugcggggCCGACGGCggcgggaccaUGUUCa- -3' miRNA: 3'- guGCGCGGGCa-------------GGUUGCCG----------GCAAGaa -5' |
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9074 | 5' | -58.9 | NC_002512.2 | + | 109106 | 0.66 | 0.877683 |
Target: 5'- cCGC-CGCCCccgCCGACGGCCGc---- -3' miRNA: 3'- -GUGcGCGGGca-GGUUGCCGGCaagaa -5' |
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9074 | 5' | -58.9 | NC_002512.2 | + | 154741 | 0.66 | 0.877683 |
Target: 5'- cCGCG-GCCgCGUCCGACGGCg--UCg- -3' miRNA: 3'- -GUGCgCGG-GCAGGUUGCCGgcaAGaa -5' |
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9074 | 5' | -58.9 | NC_002512.2 | + | 190958 | 0.66 | 0.877683 |
Target: 5'- gCGgGCGCCCGcCgCGGCGGCCccUCc- -3' miRNA: 3'- -GUgCGCGGGCaG-GUUGCCGGcaAGaa -5' |
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9074 | 5' | -58.9 | NC_002512.2 | + | 75185 | 0.66 | 0.877683 |
Target: 5'- uCGCGcCGCUCGacgCCgGACGGCCGcUCg- -3' miRNA: 3'- -GUGC-GCGGGCa--GG-UUGCCGGCaAGaa -5' |
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9074 | 5' | -58.9 | NC_002512.2 | + | 166013 | 0.66 | 0.877683 |
Target: 5'- gACG-GCUccggggacgCGUUCGGCGGCCGUUCc- -3' miRNA: 3'- gUGCgCGG---------GCAGGUUGCCGGCAAGaa -5' |
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9074 | 5' | -58.9 | NC_002512.2 | + | 209599 | 0.66 | 0.873534 |
Target: 5'- uCAC-CGCCaCGUCCAcgagauguacgaccuGCGGCCGgaCg- -3' miRNA: 3'- -GUGcGCGG-GCAGGU---------------UGCCGGCaaGaa -5' |
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9074 | 5' | -58.9 | NC_002512.2 | + | 29597 | 0.66 | 0.870728 |
Target: 5'- gACGaGCCCcaGUCCGACGGCuCGUcgUCc- -3' miRNA: 3'- gUGCgCGGG--CAGGUUGCCG-GCA--AGaa -5' |
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9074 | 5' | -58.9 | NC_002512.2 | + | 92984 | 0.66 | 0.870728 |
Target: 5'- gACGuCGCCCGacCUggUGGCCGUcUCg- -3' miRNA: 3'- gUGC-GCGGGCa-GGuuGCCGGCA-AGaa -5' |
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9074 | 5' | -58.9 | NC_002512.2 | + | 100799 | 0.66 | 0.870728 |
Target: 5'- gACGcCGCCCGUCCucGgGGUCGacCUg -3' miRNA: 3'- gUGC-GCGGGCAGGu-UgCCGGCaaGAa -5' |
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9074 | 5' | -58.9 | NC_002512.2 | + | 143221 | 0.66 | 0.870021 |
Target: 5'- uCGCGCG-CCGUCCGcucggacGCGGCCa----- -3' miRNA: 3'- -GUGCGCgGGCAGGU-------UGCCGGcaagaa -5' |
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9074 | 5' | -58.9 | NC_002512.2 | + | 92736 | 0.66 | 0.863576 |
Target: 5'- gGCGCGCUCgGUCCGAUGGCg------ -3' miRNA: 3'- gUGCGCGGG-CAGGUUGCCGgcaagaa -5' |
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9074 | 5' | -58.9 | NC_002512.2 | + | 27960 | 0.66 | 0.863576 |
Target: 5'- -cCGgGCCCGUCgCcgaugGGCGGCCGggCg- -3' miRNA: 3'- guGCgCGGGCAG-G-----UUGCCGGCaaGaa -5' |
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9074 | 5' | -58.9 | NC_002512.2 | + | 17804 | 0.66 | 0.856233 |
Target: 5'- gCACGCGCUCGUCguacaGGCGGCgGa---- -3' miRNA: 3'- -GUGCGCGGGCAGg----UUGCCGgCaagaa -5' |
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9074 | 5' | -58.9 | NC_002512.2 | + | 69421 | 0.66 | 0.856233 |
Target: 5'- aCAUGCGCUCGUgCCccgcgaAACGGCC-UUCg- -3' miRNA: 3'- -GUGCGCGGGCA-GG------UUGCCGGcAAGaa -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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