miRNA display CGI


Results 1 - 6 of 6 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
9148 5' -49.9 NC_002512.2 + 81418 1.05 0.025738
Target:  5'- uUUCCAUCCUCGUUUUUCCUCCCUUGUc -3'
miRNA:   3'- -AAGGUAGGAGCAAAAAGGAGGGAACA- -5'
9148 5' -49.9 NC_002512.2 + 171880 0.68 0.994638
Target:  5'- -gCCGUCCUCGgcg-UCC-CCCUcGUc -3'
miRNA:   3'- aaGGUAGGAGCaaaaAGGaGGGAaCA- -5'
9148 5' -49.9 NC_002512.2 + 17274 0.67 0.997158
Target:  5'- gUCCgcGUCCUCGUcg-UCCUCCUc--- -3'
miRNA:   3'- aAGG--UAGGAGCAaaaAGGAGGGaaca -5'
9148 5' -49.9 NC_002512.2 + 106552 0.67 0.997604
Target:  5'- -gUCGUCCUCGUUc-UUCUCCCgcagGUa -3'
miRNA:   3'- aaGGUAGGAGCAAaaAGGAGGGaa--CA- -5'
9148 5' -49.9 NC_002512.2 + 81581 0.67 0.996647
Target:  5'- gUCCAUCCgggaGUUUUUCuCUCCUa--- -3'
miRNA:   3'- aAGGUAGGag--CAAAAAG-GAGGGaaca -5'
9148 5' -49.9 NC_002512.2 + 104753 0.66 0.998606
Target:  5'- -gCCGUCCUCGUcgccgUCgUCCCg--- -3'
miRNA:   3'- aaGGUAGGAGCAaaa--AGgAGGGaaca -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.