Results 1 - 20 of 40 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
9150 | 5' | -56.1 | NC_002512.2 | + | 72379 | 0.66 | 0.962833 |
Target: 5'- gAUGA-UCGUCUGGUCGCgGUGgacgCUGAu -3' miRNA: 3'- gUGCUcAGUAGGCCAGCG-CACa---GGCU- -5' |
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9150 | 5' | -56.1 | NC_002512.2 | + | 211891 | 0.66 | 0.962833 |
Target: 5'- aGCGGGUCAccuUCCGGggcugcCGCGUcgUCGAg -3' miRNA: 3'- gUGCUCAGU---AGGCCa-----GCGCAcaGGCU- -5' |
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9150 | 5' | -56.1 | NC_002512.2 | + | 208132 | 0.66 | 0.968972 |
Target: 5'- uGCGAGUUcaGUCCgcagaucggagcGGUcCGCGcgGUCCGGu -3' miRNA: 3'- gUGCUCAG--UAGG------------CCA-GCGCa-CAGGCU- -5' |
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9150 | 5' | -56.1 | NC_002512.2 | + | 154331 | 0.66 | 0.968972 |
Target: 5'- --gGAGUCGUCCGcGUCGUc-GUCCu- -3' miRNA: 3'- gugCUCAGUAGGC-CAGCGcaCAGGcu -5' |
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9150 | 5' | -56.1 | NC_002512.2 | + | 116871 | 0.66 | 0.968972 |
Target: 5'- uCGCGAGUC-UgUGGUCGCGg--CUGGc -3' miRNA: 3'- -GUGCUCAGuAgGCCAGCGCacaGGCU- -5' |
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9150 | 5' | -56.1 | NC_002512.2 | + | 142243 | 0.67 | 0.943922 |
Target: 5'- aCGCGGGaUCuccgaucugggAUCCGGUCGUc-GUCCGGg -3' miRNA: 3'- -GUGCUC-AG-----------UAGGCCAGCGcaCAGGCU- -5' |
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9150 | 5' | -56.1 | NC_002512.2 | + | 206134 | 0.67 | 0.952116 |
Target: 5'- aGCGGGgCGUCCGGUCGCcagacgGUCaUGGc -3' miRNA: 3'- gUGCUCaGUAGGCCAGCGca----CAG-GCU- -5' |
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9150 | 5' | -56.1 | NC_002512.2 | + | 222504 | 0.67 | 0.952116 |
Target: 5'- aCGCGAucGUCGUCCGGa-GCGUuUCCa- -3' miRNA: 3'- -GUGCU--CAGUAGGCCagCGCAcAGGcu -5' |
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9150 | 5' | -56.1 | NC_002512.2 | + | 104080 | 0.67 | 0.952116 |
Target: 5'- -cCGucGUcCGUCCGGUCGCcGUGguugCCGGu -3' miRNA: 3'- guGCu-CA-GUAGGCCAGCG-CACa---GGCU- -5' |
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9150 | 5' | -56.1 | NC_002512.2 | + | 163444 | 0.67 | 0.952116 |
Target: 5'- uGCGAGgcggagAUCCGGUC-CGUGUCUu- -3' miRNA: 3'- gUGCUCag----UAGGCCAGcGCACAGGcu -5' |
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9150 | 5' | -56.1 | NC_002512.2 | + | 194640 | 0.67 | 0.947715 |
Target: 5'- aCACGGGgcaccCGUCCgaGGUCGaCGgaugaucUGUCCGAg -3' miRNA: 3'- -GUGCUCa----GUAGG--CCAGC-GC-------ACAGGCU- -5' |
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9150 | 5' | -56.1 | NC_002512.2 | + | 82387 | 0.67 | 0.943922 |
Target: 5'- gACGGGagAagcgCCGGUCGCGcccGUCCGc -3' miRNA: 3'- gUGCUCagUa---GGCCAGCGCa--CAGGCu -5' |
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9150 | 5' | -56.1 | NC_002512.2 | + | 5843 | 0.67 | 0.936744 |
Target: 5'- gACGAGUCGUCCccggcggccccgucgGaGUCccggGCGUcGUCCGAc -3' miRNA: 3'- gUGCUCAGUAGG---------------C-CAG----CGCA-CAGGCU- -5' |
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9150 | 5' | -56.1 | NC_002512.2 | + | 114278 | 0.67 | 0.930003 |
Target: 5'- aGCGGGUCcgCCGcGUC-CGUGUUCu- -3' miRNA: 3'- gUGCUCAGuaGGC-CAGcGCACAGGcu -5' |
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9150 | 5' | -56.1 | NC_002512.2 | + | 163233 | 0.67 | 0.930003 |
Target: 5'- cCACgGAGgag-CUGGaUCGCGUGUUCGAa -3' miRNA: 3'- -GUG-CUCaguaGGCC-AGCGCACAGGCU- -5' |
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9150 | 5' | -56.1 | NC_002512.2 | + | 94497 | 0.67 | 0.939501 |
Target: 5'- gACGcAGUCGUCgGG-CGCGUcUUCGAa -3' miRNA: 3'- gUGC-UCAGUAGgCCaGCGCAcAGGCU- -5' |
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9150 | 5' | -56.1 | NC_002512.2 | + | 100408 | 0.67 | 0.939501 |
Target: 5'- gGCGAcggCGUCCucGUCGUGUcGUCCGAg -3' miRNA: 3'- gUGCUca-GUAGGc-CAGCGCA-CAGGCU- -5' |
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9150 | 5' | -56.1 | NC_002512.2 | + | 160135 | 0.67 | 0.934862 |
Target: 5'- gGCGAGguggCgAUCCGGgcgcCGCGUGUCa-- -3' miRNA: 3'- gUGCUCa---G-UAGGCCa---GCGCACAGgcu -5' |
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9150 | 5' | -56.1 | NC_002512.2 | + | 36995 | 0.67 | 0.943922 |
Target: 5'- aCACG-G-CAUCCGGUCGCGaagUGUUgGu -3' miRNA: 3'- -GUGCuCaGUAGGCCAGCGC---ACAGgCu -5' |
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9150 | 5' | -56.1 | NC_002512.2 | + | 127010 | 0.68 | 0.908372 |
Target: 5'- --gGAGUCgGUCCGGgCGCGUuucUCCGAc -3' miRNA: 3'- gugCUCAG-UAGGCCaGCGCAc--AGGCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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